Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase.

Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase. Research Abstract Details 

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Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase. Abstract Text:

Prashani Mudika Ekanayake,Mahanama De Zoysa,Hyun-Sil Kang,Qiang Wan,Youngheun Jee,Youn-Ho Lee,Sang-Jin Kim,Jehee Lee,

Catalase is an antioxidant enzyme that plays a significant role in protection against oxidative stress by detoxification of hydrogen peroxide (H(2)O(2)). A gene coding for a putative catalase was isolated from the disk abalone (Haliotis discus discus) cDNA library and denoted as Ab-catalase. The full-length (2864bp) Ab-catalase cDNA contained 1,503bp open reading frame (ORF), encoding 501 amino acid residues with 56kDa predicted molecular weight. The deduced amino acid sequence of Ab-catalase has characteristic features of catalase family such as catalytic site motif ((61)FNRERIPERVVHAKGAG(77)), heme-ligand signature motif ((351)RLYSYSDT(358)), NADPH and heme binding residues. Phylogenetic and pairwise identity results indicated that Ab-catalase is more similar to scallop (Chlamys farreri) catalase with 80% amino acid identity except for other reported disk abalone catalase sequences. Constitutive Ab-catalase expression was detected in gill, mantle, gonad, hemocytes, abductor muscle and digestive tract in tissue specific manner. Ab-catalase mRNA was up-regulated in gill and digestive tract tissues for the first 3h post injection of H(2)O(2), showing the inducible ability of abalone catalase against oxidative stress generated by H(2)O(2). The purified recombinant catalase showed 30,000U/mg enzymatic activity against H(2)O(2) and biochemical properties of higher thermal stability and broad spectrum of pH. Our results suggest that abalone catalase may play an important role in regulating oxidative stress by scavenging H(2)O(2).

Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase. Publishing Authors By Initials

PM Ekanayake,M De Zoysa,HS Kang,Q Wan,Y Jee,YH Lee,SJ Kim,J Lee,

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Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Fish & shellfish immunology

VOLUME: 24

Page Numbers: 267-78

Journal Abbreviation: Fish Shellfish Immunol.

ISSN: 1050-4648

DAY: 22

MONTH: 11

YEAR: 2007

Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase.

AFFILIATION: Department of Marine Biotechnology, Cheju National University, 66 Jejudaehakno, Jeju 690-756, Republic of Korea.

Country: England

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MEDLINETA: Fish Shellfish Immunol

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