Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp.

Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Research Abstract Details 

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Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Abstract Text:

T Nakamura,I Yoshioka,M Takahashi,H Toh,K Izui,

The ppc gene, which encodes phosphoenolpyruvate carboxylase (PEPC) of an extreme thermophile, Thermus sp., was cloned and sequenced. The ppc gene had a high G+C content (69.2%). An open reading frame for a 857-amino-acid polypeptide was found in the gene. The calculated molecular mass was 95,632. The amino acid sequence of Thermus PEPC was 31-37% identical and 52-57% similar to those of 17 PEPCs from mesophilic organisms. No Cys residue was found in the polypeptide, demonstrating that this residue is not essential for the catalytic activity of PEPC. The cloned gene was expressed in Escherichia coli and thermostable PEPC was obtained.

Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Publishing Authors By Initials

T Nakamura,I Yoshioka,M Takahashi,H Toh,K Izui,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus research

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Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 319-24

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1995

Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Keywords Mesh Terms:

KEYWORDS: Thermus

MESH TERMS: genetics

Chemical & Substance for Abstract: Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp. Information

Substance Name: Phosphoenolpyruvate Carboxylase

Registry Number: EC 4.1.1.31

Grant and Affiliation Information for Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp.

AFFILIATION: Department of Chemistry, Faculty of Science, Kyoto University.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: D42166

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