Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Abstract Text:

J S Takagi,M Tokushige,Y Shimura,

The aspartase gene (aspA) of Pseudomonas fluorescens was cloned and the nucleotide sequence of the 2,066-base-pair DNA fragment containing the aspA gene was determined. The amino acid sequence of the protein deduced from the nucleotide sequence was confirmed by N- and C-terminal sequence analysis of the purified enzyme protein. The deduced amino acid composition also fitted the previous amino acid analysis results well (Takagi et al. (1984) J. Biochem. 96, 545-552). These results indicate that aspartase of P. fluorescens consists of four identical subunits with a molecular weight of 50,859, composed of 472 amino acid residues. The coding sequence of the gene was preceded by a potential Shine-Dalgarno sequence and by a few promoter-like structures. Following the stop codon there was a structure which is reminiscent of the Escherichia coli rho-independent terminator. The G + C content of the coding sequence was found to be 62.3%. Inspection of the codon usage for the aspA gene revealed as high as 80.0% preference for G or C at the third codon position. The deduced amino acid sequence was 56.3% homologous with that of the enzyme of E. coli W (Takagi et al. (1985) Nucl. Acids Res. 13, 2063-2074). Cys-140 and Cys-430 of the E. coli enzyme, which had been assigned as functionally essential (Ida & Tokushige (1985) J. Biochem. 98, 793-797), were substituted by Ala-140 and Ala-431, respectively, in the P. fluorescens enzyme.

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Publishing Authors By Initials

JS Takagi,M Tokushige,Y Shimura,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, nucleic acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, nucleic acid research

PUBMED ID PMID:

MEDLINE DATE:

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 697-705

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1986

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Nucleic Acid

MESH TERMS: genetics

Chemical & Substance for Abstract: Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. Information

Substance Name: Aspartate Ammonia-Lyase

Registry Number: EC 4.3.1.1

Grant and Affiliation Information for Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: X04441

Number Hits: 0

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens Related Publications

• Accuracy of Body Composition Estimated By BIA in Stroke Survivors Resembles That in Healthy Adults: 1493: Board #256 May 30 11:00 AM -12:30 PM.
• Characterization of an additional factor for amino acid polymerization from silk glands of silkworms.
• Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.
• Conversion of transfer ribonucleic acid precursors to 4 S RNA by enzymes from Bombyx mori.
• Factors involved in the activation of pre-mRNA splicing from downstream splicing enhancers.
• Fractionation and characterization of silk gland histones.
• Fractionation of glycine, alanine, and serine transfer ribonucleic acids from the silk gland.
• Fractionation of histones from calf thymus and posterior silkglands by countercurrent distribution.
• Fractionation, isolation and characterization of nonhistone chromosomal protein from calf thymus.
• Guanosine triphosphate-dependent enzymatic binding of amino-acyl-transfer ribonucleic acid to silk gland ribosomes.
• In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract.
• Initiation of fibroin biosynthesis. I. Isolation of nascent fibroin peptides from the posterior silk gland of Bombyx mori.
• Mg2+-dependent unwinding of DNA by nonhistone chromosomal protein HMG(1 + 2) from pig thymus as determined by DNA melting temperature analysis.
• Predominant synthesis of fibroin heavy and light chains on the membrane-bound polysomes prepared from the posterior silk gland of the silkworm, Bombyx mori.
• Preparation and some properties of three amino acid polymerization factors from silk gland of silkworm.
• Proceedings: Sequential processing of precursor tRNA molecules in Escherichia coli.
• Purification and some properties of G-factor from the silk gland of silkworm.
• Purification and some properties of a specific nuclease which cleaves transfer RNA precursors from the posterior silk gland of Bombyx mori.
• Sequential grouping of nonhistone chromosomal protein from pig thymus.
• Specific repression of chromatin-dependent RNA synthesis by silk gland histones.
• Stimulation of amino acid incorporation in an Escherichia coli cell-free system by silkgland RNA.
• Structural analysis of the alanine-rich, arginine-rich histone. I. Amino acid compositions and sequences of tryptic peptides.
• Studies on immunological properties of fibroin heavy and light chains.
• Studies on the biosynthesis of glycine in the silkworm. 3. Formation of glycine from C14-glycolic acid and the occurrence of glycolate oxidase.
• The biosynthesis of transfer RNA in insects. I. Increase of amino acid acceptor activity of specific tRNA's utilized for silk protein biosynthesis in the silk gland of Bombyx mori.
• The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori.
• The effect of formycin on the processing of transfer RNA precursors in the posterior silk gland of Bombyx mori.
• The interaction of elongation factor 2 with ribosomes from silk gland. Formation of an EF-2-ribosome-GDP complex.
• Three factors for amino acid polymerization from silkworm silkglands.
• Unwinding of DNA by nonhistone chromosomal protein HMG(1 + 2) from pig thymus as determined with endonuclease.