Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce.

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Abstract Text:

Mohammad Basyuni,Hirosuke Oku,Etsuko Tsujimoto,Shigeyuki Baba,

To obtain cDNAs encoding oxidosqualene cyclase (OSC), we cloned two cDNAs, KcCAS and RsCAS, from roots of Kandelia candel (L.) Druce and leaves of Rhizophora stylosa Griff. by homology based PCR method respectively. The deduced amino acid sequences of both OSCs showed 82% homology to cycloartenol synthases from Lotus japonicus (OSC5) and Ricinus cummunis (RcCAS), suggesting that these are cycloartenol synthases of K. candel and R. stylosa. The genes obtained were expressed in a lanosterol synthase deficient Saccharomyces cerevisiae (ERG7) strain, GIL77. GC-MS analysis identified the accumulated reaction product in the yeast transformant to be cycloartenol, indicating that both KcCAS and RsCAS encode cycloartenol synthase.

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Publishing Authors By Initials

M Basyuni,H Oku,E Tsujimoto,S Baba,

For similar fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research abstracts see: fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research

PUBMED ID PMID:

MEDLINE DATE:

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Bioscience, biotechnology, and biochemistry

VOLUME: 71

Page Numbers: 1788-92

Journal Abbreviation: Biosci. Biotechnol. Biochem.

ISSN: 0916-8451

DAY: 7

MONTH: 07

YEAR: 2007

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9205717

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae

MESH TERMS: genetics

Chemical & Substance for Abstract: Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce. Information

Substance Name: cycloartenol synthase

Registry Number: EC 5.4.99.8

Grant and Affiliation Information for Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce.

AFFILIATION: United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biosci Biotechnol Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff and Kandelia candel L Druce Related Publications

• Charging dynamics of the electric double layer in porous media.
• Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce.
• Comparison of intrahepatic and pancreatic perfusion on fusion images using a combined SPECT/CT system and assessment of efficacy of combined continuous arterial infusion and systemic chemotherapy in advanced pancreatic carcinoma.
• Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) blocks the chemotaxis of neutrophils by inhibiting signal transduction through IL-8 receptors.
• Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle.
• Dipole-LUMO/dipolarophile-HOMO controlled asymmetric cycloadditions of carbonyl ylides catalyzed by chiral Lewis acids.
• Effects of Salmonella enterica subsp. enterica serovar Enteritidis vaccination in layer hens subjected to S. Enteritidis challenge and various feed withdrawal regimens.
• Efficacy of etanercept in patients with AA amyloidosis secondary to rheumatoid arthritis.
• Embryogenesis and swimming behavior of Medaka fish in JUSTSAP STARS Program (STS-107).
• Experiences in quality assurance at Bawku Hospital Eye Department, Ghana.
• Gemcitabine and paclitaxel chemotherapy as a second-line treatment for advanced or metastatic urothelial carcinoma.
• InCl3/I2-catalyzed cross-coupling of alkyl trimethylsilyl ethers and allylsilanes via an in situ derived combined Lewis acid of InCl3 and Me3SiI.
• Induction of interleukin-8 (CXCL-8) by tumor necrosis factor-alpha and leukemia inhibitory factor in pancreatic carcinoma cells: Impact of CXCL-8 as an autocrine growth factor.
• Low-group-velocity and low-dispersion slow light in photonic crystal waveguides.
• Ossification of the posterior longitudinal ligament of the cervical spine: histopathological findings around the calcification and ossification front.
• Pituitary adenylate cyclase-activating polypeptide is associated with schizophrenia.
• Posterior 360-degree stabilisation of the upper thoracic spine: a technical note.
• Potent and selective inhibition of Tat-dependent HIV-1 replication in chronically infected cells by a novel naphthalene derivative JTK-101.
• Predictive factors for platelet increase after partial splenic embolization in liver cirrhosis patients.
• Prognostic relevance of activated Akt kinase in node-negative breast cancer: a clinicopathological study of 99 cases.
• Quantitative gait analysis of patients with bilateral hip osteoarthritis excluding the influence of walking speed.
• Sensory aphasia after closed head injury.
• Single-molecule measurements with a single quantum dot.
• The red eye - first aid at the primary level.
• Thoracic ossification of the human ligamentum flavum: histopathological and immunohistochemical findings around the ossified lesion.
• X-ray absorption beamline at the Mitsubishi compact storage ring.
• [Circadian rhythm monitoring in an individual animal]
• [High-normal blood pressure]
• [Molecular pharmacologic approaches to functional analysis of new biological target molecules for drug discovery]
• [Studies on chemical constituents from roots of Peucedanum praeruptorum II]