Cloning and Detecting Signature MicroRNAs from Mammalian Cells.

Cloning and Detecting Signature MicroRNAs from Mammalian Cells. Research Abstract Details 

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Cloning and Detecting Signature MicroRNAs from Mammalian Cells. Abstract Text:

Guihua Sun,Haitang Li,John J Rossi,

MicroRNAs (miRNAs) are about 19- to 24-nucleotides long noncoding regulatory small RNAs that could silence target gene expression through base pairing to the complementary sequences in the 3' untranslated region (3'UTR) of targeted genes. They are evolutionally conserved and play an important regulatory role in embryogenesis, cell differentiation, and proliferation. They are also involved in pathogenesis and progression of some human diseases. There are about 1000 human miRNAs predicted today, and it is estimated that they could target about 30% of all human transcripts. Profiling the miRNAs that are expressed in the experimental cells became an important issue as different cells express different signature miRNAs or express the same miRNAs at different level. Small RNA cloning is a reliable way to characterize those tissue- or cell-specific signature miRNAs. This chapter describes a relatively nonlaborious polyadenylation-mediated complementary DNA (cDNA) cloning method that will identify most of the small RNAs expressed in the cells of interest. This procedure can also be used to verify bioinformatic predictions of miRNAs/small interfering RNAs (siRNAs) as well as to identify new miRNAs/siRNAs.

Cloning and Detecting Signature MicroRNAs from Mammalian Cells. Publishing Authors By Initials

G Sun,H Li,JJ Rossi,

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Cloning and Detecting Signature MicroRNAs from Mammalian Cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in enzymology

VOLUME: 427

Page Numbers: 123-38

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 27

MONTH: 08

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Cloning and Detecting Signature MicroRNAs from Mammalian Cells.

AFFILIATION: Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, California; Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California.

Country: United States

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MEDLINETA: Methods Enzymol

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