Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.

Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Research Abstract Details 

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Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Abstract Text:

We report the cloning and characterization of a full-length cDNA encoding the human cytoskeletal isoform of alpha-actinin (alpha A), a ubiquitous actin-binding protein that shares structural homology with spectrin and dystrophin. The gene encodes 891 amino acids with 96%-98% sequence identity at the amino acid level to chicken nonskeletal muscle alpha A. Transient expression in COS cells produces a protein of approximately 104 kD that comigrates on SDS-PAGE with native alpha A. This alpha A gene is localized to chromosome 14q22-q24 by somatic cell hybrid and in situ hybridization analyses. Pulsed-field gel analysis of human genomic DNA revealed identically sized fragments when cDNA probes for alpha A and erythroid beta-spectrin were used; the latter gene has been previously localized to chromosome 14, band q22. These observations indicate that the genes for cytoskeletal alpha A and beta-spectrin are, in all likelihood, closely physically linked and that, in accordance with their similar structural features, they arose by partial duplication of an ancestral gene.

Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Publishing Authors By Initials

For similar proteins: blood proteins: spectrin research abstracts see: proteins: blood proteins: spectrin research

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Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of human genetics

VOLUME: 47

Page Numbers: 62-71

Journal Abbreviation: Am. J. Hum. Genet.

ISSN: 0002-9297

DAY: 3

MONTH: Jul

YEAR: 1990

Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Information

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LANGUAGE: eng

NlmUniqueID: 370475

Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Keywords Mesh Terms:

KEYWORDS: Spectrin

MESH TERMS: genetics

Chemical & Substance for Abstract: Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Information

Substance Name: Spectrin

Registry Number: 12634-43-4

Grant and Affiliation Information for Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.

AFFILIATION: Whitehead Institute for Biomedical Research, Cambridge, MA.

Country: UNITED STATES

AGENCY: United States NHLBI

GRANT: HL02277

ACRONYM: HL

MEDLINETA: Am J Hum Genet

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ACCESSION NUMBER: M31300

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