Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana.

Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana. Research Abstract Details 

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Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana. Abstract Text:

Sichun Zheng,Huimin Deng,Tim Ladd,Bill L Tomkins,Peter J Krell,Qili Feng,Sichun Zheng,Huimin Deng,Tim Ladd,Bill L Tomkins,Peter J Krell,Qili Feng,

Two Choristoneura fumiferana glutathione S-transferase cDNAs were cloned from a cDNA library constructed using mRNA from the midgut cell line, CF-203. These cDNAs (CfGST2, CfGST3) encoded two structurally different proteins with a predicted molecular mass of 21 and 24 kDa, respectively, which was confirmed through protein expression in a bacterial system. Quantitative reverse-transcription PCR analyses revealed that the transcripts of these two genes were present in the epidermis, fat body, and midgut of the 6th instar larvae. CfGST2 was expressed in the fat body when the insects were close to pupal molting, while it was constantly expressed in the other two tissues during the 6th instar stage. CfGST3 gene was expressed highly and constantly in all of the tissues throughout the 6th instar stage. Immunohistochemistry analysis demonstrated that CfGST2 and CfGST3 proteins were present mainly in the fat body and epidermis and no protein was detected in the midgut. CfGST2 and CfGST3 were different from CfGST reported before (Feng et al., 1999: Insect Biochem Mol Biol 29:779-793) in amino acid sequence, expression pattern, and responsiveness to tebufenozide.

Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana. Publishing Authors By Initials

S Zheng,H Deng,T Ladd,BL Tomkins,PJ Krell,Q Feng,S Zheng,H Deng,T Ladd,BL Tomkins,PJ Krell,Q Feng,

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Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Archives of insect biochemistry and physiology

VOLUME: 66

Page Numbers: 146-57

Journal Abbreviation: Arch. Insect Biochem. Physiol.

ISSN: 0739-4462

DAY: 1

MONTH: Nov

YEAR: 2007

Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana. Information

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LANGUAGE: eng

NlmUniqueID: 8501752

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Grant and Affiliation Information for Cloning and characterization of two glutathione S-transferase cDNAs in the spruce budworm, Choristoneura fumiferana.

AFFILIATION: Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China.

Country: United States

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MEDLINETA: Arch Insect Biochem Physiol

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