Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola.

Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. Research Abstract Details 

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Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. Abstract Text:

Inge N A Van Bogaert,Dirk Develter,Wim Soetaert,Erick J Vandamme,

Candida bombicola is a yeast with at least two appealing features. The species can grow on alkanes when provided as the sole carbon source, and it produces glycolipids, which have several industrial, cosmetic and pharmaceutical applications. Both metabolic processes require in their pathway the activity of cytochrome P450 monooxygenase. This enzyme needs and gets reducing equivalents from NADPH cytochrome P450 reductase (CPR). The CPR gene of Candida bombicola was isolated using degenerate PCR and genomic walking. The gene encodes an enzyme of 687 amino acids, which shows homology with known CPRs of other species. The functionality of the gene was proven by heterologous expression in Escherichia coli. The recombinant protein exhibited NADPH-dependent cytochrome c reducing activity. Cloning and characterization of this enzyme is an important step in the study of the cytochrome P450 monooxygenase system of Candida bombicola. The GenBank accession number of the sequence described in this article is EF050789.

Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. Publishing Authors By Initials

IN Van Bogaert,D Develter,W Soetaert,EJ Vandamme,

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Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS yeast research

VOLUME: 7

Page Numbers: 922-8

Journal Abbreviation: FEMS Yeast Res.

ISSN: 1567-1356

DAY: 7

MONTH: 06

YEAR: 2007

Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola.

AFFILIATION: Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. inge.vanbogaert@ugent.be

Country: England

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MEDLINETA: FEMS Yeast Res

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