Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene.

Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Research Abstract Details 

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Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Abstract Text:

M Okabe,T Yakushi,Y Asai,M Homma,

Four motor proteins, MotX, MotY, PomA, and PomB, have been identified as constituents of the Na(+)-driven flagellum of Vibrio species. In this study, the complete motX gene was cloned from Vibrio alginolyticus and shown to complement three mot mutations, motX94, motX115, and motX119, as well as a V. parahaemolyticus motX mutant. The motX94 mutant contains a frameshift at Val86 of MotX, while the motX115 and motX119 mutations comprise substitutions of Ala146 to Val and Gln 194 to amber, respectively. When MotX was overexpressed in Vibrio cells, the amount of MotY detected in the membrane fraction increased, and vice versa, suggesting that MotX and MotY mutually stabilize each other by interacting at the membrane level. When a plasmid containing the motX gene was introduced into motY mutants NMB117 (motY117) and VIO542 (motY542), the mutations were suppressed. In contrast, motY could not cause the recovery of any swarm-defective motX mutants studied. Considering the above evidence, we propose that MotX is more directly involved than MotY in the mechanical functioning of the Na(+)-type flagellar motor, and that MotY may stabilize MotX to support its interaction with other Mot proteins.

Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Publishing Authors By Initials

M Okabe,T Yakushi,Y Asai,M Homma,

For similar bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: vibrionaceae: vibrio research abstracts see: bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: vibrionaceae: vibrio research

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Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 879-84

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 2001

Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Keywords Mesh Terms:

KEYWORDS: Vibrio

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. Information

Substance Name: Sodium

Registry Number: 7440-23-5

Grant and Affiliation Information for Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene.

AFFILIATION: Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.

Country: Japan

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MEDLINETA: J Biochem

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