Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe.

Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Research Abstract Details 

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Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Abstract Text:

Peter M Piermarini,Inyeong Choi,Walter F Boron,

The squid giant axon is a classic model system for understanding both excitable membranes and ion transport. To date, a Na(+)-driven Cl-HCO(3)(-) exchanger, sqNDCBE--related to the SLC4 superfamily and cloned from giant fiber lobe cDNA--is the only HCO(3)(-)-transporting protein cloned and characterized from a squid. The goal of our study was to clone and characterize another SLC4-like cDNA. We used degenerate PCR to obtain a partial cDNA clone (squid fiber clone 3, SF3), which we extended in both the 5' and 3' directions to obtain the full-length open-reading frame. The predicted amino-acid sequence of SF3 is similar to sqNDCBE, and a phylogenetic analysis of the membrane domains indicates that SF3 clusters with electroneutral Na(+)-coupled SLC4 transporters. However, when we measure pH(i) and membrane potential--or use two-electrode voltage clamping to measure currents--on Xenopus oocytes expressing SF3, the oocytes exhibit the characteristics of an electrogenic Na/HCO(3)(-) cotransporter, NBCe. That is, exposure to extracellular CO(2)/HCO(3)(-) not only causes a fall in pH(i), followed by a robust recovery, but also causes a rapid hyperpolarization. The current-voltage relationship is also characteristic of an electrogenic NBC. The pH(i) recovery and current require HCO(3)(-) and Na(+), and are blocked by DIDS. Furthermore, neither K(+) nor Li(+) can fully replace Na(+) in supporting the pH(i) recovery. Extracellular Cl(-) is not necessary for the transporter to operate. Therefore, SF3 is an NBCe, representing the first NBCe characterized from an invertebrate.

Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Publishing Authors By Initials

PM Piermarini,I Choi,WF Boron,

For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus research

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Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Cell physiology

VOLUME: 292

Page Numbers: C2032-45

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 31

MONTH: 01

YEAR: 2007

Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Keywords Mesh Terms:

KEYWORDS: Xenopus

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe. Information

Substance Name: Sodium-Bicarbonate Symporters

Registry Number: 0

Grant and Affiliation Information for Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe.

AFFILIATION: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA. pmp26@cornell.edu

Country: United States

AGENCY: United States NINDS

GRANT: NS18400

ACRONYM: NS

MEDLINETA: Am J Physiol Cell Physiol

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