Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family.

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Abstract Text:

V M Paralkar,A L Vail,W A Grasser,T A Brown,H Xu,S Vukicevic,H Z Ke,H Qi,T A Owen,D D Thompson,

Members of the transforming growth factor-beta (TGF-beta) superfamily of growth and differentiation factors have been identified in a wide variety of organisms, ranging from invertebrates to mammals. Bone morphogenetic proteins (BMPs) constitute a subgroup of proteins belonging to the TGF-beta superfamily. BMPs were initially identified by their ability to induce endochondral bone formation at ectopic sites, suggesting a critical role for this family in development and regeneration of the skeleton. They are also expressed at a variety of nonskeletal sites during development, suggesting possible extraskeletal roles for these proteins. We cloned a novel member of the BMP family that is expressed at high levels in the placenta and the prostate and that we have designated as prostate-derived factor (PDF). Based on cDNA sequence analysis, the predicted PDF protein contains two cysteines in addition to the seven conserved cysteines that are the hallmark of the members of the TGF-beta superfamily. In addition, Northern blot hybridization to poly(A)+ RNA showed low levels of expression in the kidney and pancreas. We further characterized the expression of this member of the BMP family by in situ hybridization and immunohistochemistry. These results show high expression in the terminal villae of the placenta. The expression of the protein as visualized by immunohistochemistry shows an expression pattern identical to that of the message in the terminal villae of the placenta. In day 18 rat embryos, protein expression was also seen in the skin and in the cartilaginous tissue of developing skeleton. Orchidectomy and dihydrotestosterone treatment of rats revealed that PDF expression is regulated by androgens in the prostate. In addition, subcutaneous implantation of recombinant PDF induced cartilage formation and the early stages of endochondral bone formation. These data indicate that PDF has a functional relationship to the BMPs.

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Publishing Authors By Initials

VM Paralkar,AL Vail,WA Grasser,TA Brown,H Xu,S Vukicevic,HZ Ke,H Qi,TA Owen,DD Thompson,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 273

Page Numbers: 13760-7

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 29

MONTH: May

YEAR: 1998

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family. Information

Substance Name: growth differentiation factor 15

Registry Number: 0

Grant and Affiliation Information for Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family.

AFFILIATION: Department of Metabolic Diseases, Central Research Division, Pfizer, Inc., Groton, Connecticut 06340, USA. Vishwas_M_Paralkar@groton.pfizer.com

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: AF003934

Number Hits: 0

Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family Related Publications

• A preliminary study of growth, serum prolactin and reproductive performance of beef heifers grazing Acremonium coenophialum -infected tall fescue.
• An assessment of reproductive wastage in sheep.
• Analysis method for measuring submicroscopic distances with blinking quantum dots.
• Calibration and Application of an X-ray Image Intensifier/ Charge-Coupled Device Detector for Monochromatic Macromolecular Crystallography.
• Cardiovascular dysfunction in dogs associated with critical illnesses.
• Changes in health for the uninsured after reaching age-eligibility for Medicare.
• Decremental effects of context exposure following delay eyeblink conditioning in rabbits.
• Developmental ability of in vitro matured sheep oocytes collected during the nonbreeding season and fertilized in vitro with frozen ram semen.
• Dynamic mapping of normal human hippocampal development.
• Effect of calf removal on serum luteinizing hormone and cortisol concentrations in postpartum beef cows.
• Effect of fluid irrigation on core body temperature during laparoscopic common bile duct exploration.
• Effect of suckling on cortisol, progesterone and luteinizing hormone in postpartum beef cows.
• Effects of progesterone or estradiol on uterine tubal transport of ova in the cow.
• Flare Associated with LHRH-Agonist Therapy.
• Identification and characterization of RTVP1/GLIPR1-like genes, a novel p53 target gene cluster.
• Improving estimates of genetic maps: a maximum likelihood approach.
• Influence of mineral supplementation on bovine serum, liver and endometrium at Day 1 and Day 12 of the estrous cycle.
• Multipoint linkage analysis with many multiallelic or dense diallelic markers: Markov chain-Monte Carlo provides practical approaches for genome scans on general pedigrees.
• Muscle glycogen repletion in 3 breeds of young cattle is not affected by energy intake.
• Nautiloid growth rhythms and lunar dynamics (reply).
• Predictors of prolonged dysphagia following acute stroke.
• Preventing injuries in rural Canada.
• Prostate cancer detection strategies.
• Semantic distance norms computed from an electronic dictionary (WordNet).
• Spermatogenesis, testicular composition and the concentration of testosterone in the equine testis as influenced by season.
• Synthesis, characterization, and pH-triggered dethreading of alpha-cyclodextrin-poly(ethylene glycol) polyrotaxanes bearing cleavable endcaps.
• TICKS (IXODOIDEA) OF MEDICAL AND VETERINARY INTEREST FROM BIRDS IN THE BRITISH ISLES.
• The future of antidepressant pharmacotherapy.
• Zinc depletion and complications of surgery.
• rag genes: novel components of the RamR regulon that trigger morphological differentiation in Streptomyces coelicolor.