Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter.

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Abstract Text:

The glyceraldehyde-3-phosphate dehydrogenase gene (GPD) of the sophorolipid producing yeast Candida bombicola was isolated using degenerated PCR and genome walking. The obtained 3,740 bp contain the 1,008 bases of the coding sequence and 1,613 and 783 bp of the upstream and downstream regions, respectively. The corresponding protein shows high homology to the other known GPD genes and is 74% identical to the gyceraldehyde-3-phosphate dehydrogenase of Yarrowia lipolytica. The particular interest in the C. bombicola GPD gene sequence originates from the potential use of its promoter for high and constitutive expression of homologous and heterologous genes. Southern blot analysis did not give any indication for the presence of multiple GPD genes and it can therefore be expected that the promoter can be used for efficient and high expression. This hypothesis was further confirmed by the biased codon usage in the GPD gene. GDP promoter fragments of different lengths were used to construct hygromycin resistance cassettes. The constructs were used for the transformation of C. bombicola and all of them, even the ones with only 190 bp of the GPD promoter, were able to render the cells resistant to hygromycin. The efficacy of a short GPD promoter can be a convenient characteristic for the construction of compact expression cassettes or vectors for C. bombicola. The GenBank accession number of the sequence described in this article is EU315245.

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of industrial microbiology & biotechnology

VOLUME: 35

Page Numbers: 1085-92

Journal Abbreviation: J. Ind. Microbiol. Biotechnol.

ISSN: 1367-5435

DAY: 2

MONTH: 07

YEAR: 2008

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9705544

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter.

AFFILIATION: Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium, Inge.VanBogaert@UGent.be.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Ind Microbiol Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter Related Publications

• 'No willpower required'. The slimming industry and the right to sell dreams.
• A Computer-aided noninterfering on-line technique for monitoring oxygen-transfer characteristics during fermentation processes.
• Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.
• Burkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia.
• Burkholderia sartisoli sp. nov., isolated from a polycyclic aromatic hydrocarbon-contaminated soil.
• Cerulenin inhibits de novo sophorolipid synthesis of Candida bombicola.
• Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter.
• Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola.
• Cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene and the use of its promoter for expression in Myrothecium gramineum, a novel expression host.
• Collimonas arenae sp. nov. and Collimonas pratensis sp. nov., isolated from (semi-)natural grassland soils.
• Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli.
• Comparison of different strategies to reduce acetate formation in Escherichia coli.
• Comparison of protein quantification and extraction methods suitable for E. coli cultures.
• Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli.
• Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola.
• Devriesea agamarum gen. nov., sp. nov., a novel actinobacterium associated with dermatitis and septicaemia in agamid lizards.
• Estimation of an in vivo fitness landscape experienced by HIV-1 under drug selective pressure useful for prediction of drug resistance evolution during treatment.
• Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species.
• Matrix-assisted laser desorption ionisation-time-of of-flight mass spectrometry of intact cells allows rapid identification of Burkholderia cepacia complex.
• Minimizing acetate formation in E. coli fermentations.
• Modelled in vivo HIV fitness under drug selective pressure and estimated genetic barrier towards resistance are predictive for virological response.
• Phenotypic and molecular assessment of antimicrobial resistance in Lactobacillus paracasei strains of food origin.
• Rapid isolation of fungal genomic DNA suitable for long distance PCR.
• Renal replacement therapy in acute renal failure in children.
• The adaptation of the facade sound insulation requirements in the Belgian standard.
• The epidemic history of hepatitis C among injecting drug users in Flanders, Belgium.
• The genus Gluconobacter oxydans: comprehensive overview of biochemistry and biotechnological applications.
• Understanding the Changing Prevalence of K65R (Hot News).