Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5).

Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Research Abstract Details 

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Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Abstract Text:

D Meezaman,P Charles,E Daskal,M H Polymeropoulos,B M Martin,M C Rose,

Two unique nucleotide probes for human tracheobronchial mucin glycoprotein (TBM) were generated via polymerase chain reaction with degenerate primers deduced from the TBM:TR-3A tryptic peptide sequence and were used to isolate a 3.6 kilobase cDNA, clone NP3a, from a human nasal polyp cDNA library. Clone NP3a was localized to chromosome 11 and contained a 3168 nucleotide open reading frame which encoded three TBM peptide fragments, thus confirming that clone NP3a partially encodes TBM. TBM also contains five tandem repeats of TTVGP/S and an octapeptide GQCGTCTN, which is conserved in human intestinal mucin MUC2 and rat intestinal mucin-like protein (MLP) suggesting that this sequence has a functional significance for secreted mucins. TBM has amino acid similarity to the cysteine-rich domains at the carboxyl termini of MUC2, rat MLP, bovine and porcine submaxillary mucins, and human von Willebrand factor. Strikingly, a large percentage of the cysteine residues in the overlaps are highly conserved: 90% in MUC2 and von Willebrand factor, 80% of bovine submaxillary mucin, 70% in porcine submaxillary mucin, and 64% in rat MLP, suggesting that conserved cysteines may be important for the tertiary structure of secreted glycoproteins. These studies demonstrate that clone NP3a is a candidate for MUC5, making it the only human mucin gene reported to date whose gene product has been isolated from airway secretions.

Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Publishing Authors By Initials

D Meezaman,P Charles,E Daskal,MH Polymeropoulos,BM Martin,MC Rose,

For similar respiratory system: trachea research abstracts see: respiratory system: trachea research

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Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 269

Page Numbers: 12932-9

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 29

MONTH: Apr

YEAR: 1994

Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Keywords Mesh Terms:

KEYWORDS: Trachea

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5).

AFFILIATION: Children's Research Institute, Children's National Medical Center, Washington, D.C. 20010.

Country: UNITED STATES

AGENCY: United States NHLBI

GRANT: HL 33152

ACRONYM: HL

MEDLINETA: J Biol Chem

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