Cloned transgenic swine via in vitro production and cryopreservation.

Cloned transgenic swine via in vitro production and cryopreservation. Research Abstract Details 

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Cloned transgenic swine via in vitro production and cryopreservation. Abstract Text:

Rongfeng Li,Liangxue Lai,David Wax,Yanhong Hao,Clifton N Murphy,August Rieke,Melissa Samuel,Mike L Linville,Scott W Korte,Rhobert W Evans,James R Turk,Jing X Kang,William T Witt,Yifan Dai,Randall S Prather,

It has been notoriously difficult to successfully cryopreserve swine embryos, a task that has been even more difficult for in vitro-produced embryos. The first reproducible method of cryopreserving in vivo-produced swine embryos was after centrifugation and removal of the lipids. Here we report the adaptation of a similar process that permits the cryopreservation of in vitro-produced somatic cell nuclear transfer (SCNT) swine embryos. These embryos develop to the blastocyst stage and survive cryopreservation. Transfer of 163 cryopreserved SCNT embryos to two surrogates produced 10 piglets. Application of this technique may permit national and international movement of cloned transgenic swine embryos, storage until a suitable surrogate is available, or the long-term frozen storage of valuable genetics.

Cloned transgenic swine via in vitro production and cryopreservation. Publishing Authors By Initials

R Li,L Lai,D Wax,Y Hao,CN Murphy,A Rieke,M Samuel,ML Linville,SW Korte,RW Evans,JR Turk,JX Kang,WT Witt,Y Dai,RS Prather,

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Cloned transgenic swine via in vitro production and cryopreservation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biology of reproduction

VOLUME: 75

Page Numbers: 226-30

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ISSN: 0006-3363

DAY: 3

MONTH: 05

YEAR: 2006

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LANGUAGE: eng

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Grant and Affiliation Information for Cloned transgenic swine via in vitro production and cryopreservation.

AFFILIATION: Division of Animal Science, University of Missouri-Columbia, Columbia, Missouri 65211, USA.

Country: United States

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MEDLINETA: Biol Reprod

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