[Clone and expression of murine BTLA extracellular domain gene and its effect on the expression of B7 on dendritic cells]

[Clone and expression of murine BTLA extracellular domain gene and its effect on the expression of B7 on dendritic cells] Research Abstract Details 

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[Clone and expression of murine BTLA extracellular domain gene and its effect on the expression of B7 on dendritic cells] Abstract Text:

Jun-fa Xu,Kun Zhao,Bao-jun Huang,Ping Xiong,Min Fang,Guan-xin Shen,Fei-li Gong,

AIM: To study the regulatory effect of recombinant glutathione S-transferase (GST)-extracellular domain of murine B and T lymphocyte attenuator (mBTLAext) fusion protein GST-mBTLAext on the expression of B7 on murine dendritic cell (DC) line DC2.4. METHODS: cDNA of mBTLA was amplified from total RNA of murine splenocytes by RT-PCR. The recombinant prokaryotic expression vector pGEX-4T-2/mBTLAext was constructed by cloning mBTLAext into pGEX-4T-2 and then transformed into E. coli BL21 (DE3). The fusion protein GST-mBTLAext was expressed under the induction of 1 mmol/L IPTG, and then was extracted from inclusion body and purified through Glutathione Sepharose 4B chromatography column. The fusion protein was added into the culture supernatant of DC2.4 and its effect on the expression of B7-1 and B7-2 on DC2.4 was analyzed by flow cytometry. RESULTS: The recombinant prokaryotic expression vector pGEX-4T-2/mBTLAext was constructed and the fusion protein GST-mBTLAext was expressed successfully. The molecular weight of the fusion protein was 43.0 kDa, determined by SDS-PAGE, which was corresponding to expectation. GST-mBTLAext could up-regulate the expression of B7-1, but didn't alter the expression of B7-2, on DC2.4 in a dose dependent manner. CONCLUSION: BTLA had a regulatory effect on the expression of B7 on DC. It is significant to study the effect of BTLA on the biological behaviour of DC and its molecular mechanism.

[Clone and expression of murine BTLA extracellular domain gene and its effect on the expression of B7 on dendritic cells] Publishing Authors By Initials

JF Xu,K Zhao,BJ Huang,P Xiong,M Fang,GX Shen,FL Gong,

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[Clone and expression of murine BTLA extracellular domain gene and its effect on the expression of B7 on dendritic cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 22

Page Numbers: 413-6

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 29

MONTH: Jul

YEAR: 2006

[Clone and expression of murine BTLA extracellular domain gene and its effect on the expression of B7 on dendritic cells] Information

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LANGUAGE: chi

NlmUniqueID: 101139110

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AFFILIATION: Department of Immunology, Tongji Medical College, Huazhong Universite of Science and Technology, Wuhan 430030, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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