Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing.

Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Research Abstract Details 

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Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Abstract Text:

J Y Lee,Z Qu-Petersen,B Cao,S Kimura,R Jankowski,J Cummins,A Usas,C Gates,P Robbins,A Wernig,J Huard,

Several recent studies suggest the isolation of stem cells in skeletal muscle, but the functional properties of these muscle-derived stem cells is still unclear. In the present study, we report the purification of muscle-derived stem cells from the mdx mouse, an animal model for Duchenne muscular dystrophy. We show that enrichment of desmin(+) cells using the preplate technique from mouse primary muscle cell culture also enriches a cell population expressing CD34 and Bcl-2. The CD34(+) cells and Bcl-2(+) cells were found to reside within the basal lamina, where satellite cells are normally found. Clonal isolation and characterization from this CD34(+)Bcl-2(+) enriched population yielded a putative muscle-derived stem cell, mc13, that is capable of differentiating into both myogenic and osteogenic lineage in vitro and in vivo. The mc13 cells are c-kit and CD45 negative and express: desmin, c-met and MNF, three markers expressed in early myogenic progenitors; Flk-1, a mouse homologue of KDR recently identified in humans as a key marker in hematopoietic cells with stem cell-like characteristics; and Sca-1, a marker for both skeletal muscle and hematopoietic stem cells. Intramuscular, and more importantly, intravenous injection of mc13 cells result in muscle regeneration and partial restoration of dystrophin in mdx mice. Transplantation of mc13 cells engineered to secrete osteogenic protein differentiate in osteogenic lineage and accelerate healing of a skull defect in SCID mice. Taken together, these results suggest the isolation of a population of muscle-derived stem cells capable of improving both muscle regeneration and bone healing.

Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Publishing Authors By Initials

JY Lee,Z Qu-Petersen,B Cao,S Kimura,R Jankowski,J Cummins,A Usas,C Gates,P Robbins,A Wernig,J Huard,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of cell biology

VOLUME: 150

Page Numbers: 1085-100

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 4

MONTH: Sep

YEAR: 2000

Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing.

AFFILIATION: Growth and Development Laboratory, Department of Orthopaedic Surgery, Children's Hospital and University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.

Country: UNITED STATES

AGENCY: United States NIAMS

GRANT: 1PO1 AR45925-01

ACRONYM: AR

MEDLINETA: J Cell Biol

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