[Clinical significance of detection of AML1/ETO fusion transcripts in childhood AML using real-time quantitative reverse transcription polymerase chain reaction]

[Clinical significance of detection of AML1/ETO fusion transcripts in childhood AML using real-time quantitative reverse transcription polymerase chain reaction] Research Abstract Details 

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[Clinical significance of detection of AML1/ETO fusion transcripts in childhood AML using real-time quantitative reverse transcription polymerase chain reaction] Abstract Text:

Cai-Feng Liu,Gui-Lan Liu,Le-Ping Zhang,Yi-Fei Cheng,Ai-Dong Lu,Kai-Gong Tian,Yan-Rong Liu,Ya-Zhen Qin,

This study was aimed to investigate the clinical value of quantification of AML1/ETO fusion transcripts using real-time reverse transcription PCR. Fourteen AML1/ETO positive children out of 52 AML children were selected. A serial dilution of AML1/ETO plasmid was used as a template for the AML1/ETO real-time PCR. AML1/ETO was quantified according to the expression of the GAPDH housekeeping gene at new diagnosis and during/after chemotherapy and transplantation. SPSS statistics was used to analyze the data. The results showed that the ratio of AML1/ETO: GAPDH expression level at new diagnosis varied in the range 0.219-2.080 (median 0.648) among the patients, without relevance with percentage of blasts. The detection sensitivity was up to the dilution of 1:10(5). Six patients showed a slight decline of AML1/ETO (higher than 5 x 10(-2)) at 1 month, three of whom relapsed in the early stage and one later. Five patients had a higher level than 5 x 10(-3) at 3 months, three of whom relapsed. Four patients with always a higher level than 5 x 10(-3) all relapsed in early stage. After six months, four out of them with constant low-level expression (10(-4) - 10(-6)) were in continuous complete hematological remission (CCR). In another patient, a rapid rise of AML1/ETO transcripts could be detected at CR stage and he relapsed 5 months later. The AML1/ETO gene expression leveling off by 10(-5) - 10(-6) could be detected in 3 patients at their complete remission after 9 months. It is concluded that real-time RT-PCR is a suitable approach for quantifying AML1/ETO transcripts in monitoring of AML patients with t(8;21) during/after chemotherapy and provides data of diagnostic relevance.

[Clinical significance of detection of AML1/ETO fusion transcripts in childhood AML using real-time quantitative reverse transcription polymerase chain reaction] Publishing Authors By Initials

CF Liu,GL Liu,LP Zhang,YF Cheng,AD Lu,KG Tian,YR Liu,YZ Qin,

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[Clinical significance of detection of AML1/ETO fusion transcripts in childhood AML using real-time quantitative reverse transcription polymerase chain reaction] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing

VOLUME: 13

Page Numbers: 76-82

Journal Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za

ISSN: 1009-2137

DAY: 7

MONTH: Feb

YEAR: 2005

[Clinical significance of detection of AML1/ETO fusion transcripts in childhood AML using real-time quantitative reverse transcription polymerase chain reaction] Information

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LANGUAGE: chi

NlmUniqueID: 101084424

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AFFILIATION: Department of Pediatrics, People Hospital, Peking University, Beijing 100044, China. caifengliu24@yahoo.com.cn

Country: China

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MEDLINETA: Zhongguo Shi Yan Xue Ye Xue Za

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