Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury.

Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Research Abstract Details 

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Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Abstract Text:

Jose A Pineda,Stephen B Lewis,Alex B Valadka,Linda Papa,H Julia Hannay,Shelley C Heaton,Jason A Demery,Ming Cheng Liu,Jada M Aikman,Veronica Akle,Gretchen M Brophy,Joseph J Tepas,Kevin K W Wang,Claudia S Robertson,Ronald L Hayes,

Following traumatic brain injury (TBI), the cytoskeletal protein alpha-II-spectrin is proteolyzed by calpain and caspase-3 to signature breakdown products. To determine whether alpha -II-spectrin proteolysis is a potentially reliable biomarker for TBI in humans, the present study (1) examined levels of spectrin breakdown products (SBDPs) in cerebrospinal fluid (CSF) from adults with severe TBI and (2) examined the relationship between these levels, severity of injury, and clinical outcome. This prospective case control study enrolled 41 patients with severe TBI, defined by a Glasgow Coma Scale (GCS) score of < or =8, who underwent intraventricular intracranial pressure monitoring. Patients without TBI requiring CSF drainage for other medical reasons served as controls. Ventricular CSF was sampled from each patient at 6, 12, 24, 48, 72, 96, and 120 h following TBI and analyzed for SBDPs. Outcome was assessed using the Glasgow Outcome Score (GOS) 6 months after injury. Calpain and caspase-3 mediated SBDP levels in CSF were significantly increased in TBI patients at several time points after injury, compared to control subjects. The time course of calpain mediated SBDP150 and SBDP145 differed from that of caspase-3 mediated SBDP120 during the post-injury period examined. Mean SBDP densitometry values measured early after injury correlated with severity of injury, computed tomography (CT) scan findings, and outcome at 6 months post-injury. Taken together, these results support that alpha -II-spectrin breakdown products are potentially useful biomarker of severe TBI in humans. Our data further suggests that both necrotic/oncotic and apoptotic cell death mechanisms are activated in humans following severe TBI, but with a different time course after injury.

Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Publishing Authors By Initials

JA Pineda,SB Lewis,AB Valadka,L Papa,HJ Hannay,SC Heaton,JA Demery,MC Liu,JM Aikman,V Akle,GM Brophy,JJ Tepas,KK Wang,CS Robertson,RL Hayes,

For similar proteins: blood proteins: spectrin research abstracts see: proteins: blood proteins: spectrin research

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Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of neurotrauma

VOLUME: 24

Page Numbers: 354-66

Journal Abbreviation: J. Neurotrauma

ISSN: 0897-7151

DAY: 3

MONTH: Feb

YEAR: 2007

Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8811626

Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Keywords Mesh Terms:

KEYWORDS: Spectrin

MESH TERMS: cerebrospinal fluid

Chemical & Substance for Abstract: Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. Information

Substance Name: Caspase 3

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Clinical significance of alphaII-spectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury.

AFFILIATION: Center for Traumatic Brain Injury Studies, E.F and W.L. McKnight Brain Institute of the University of Florida, Gainesville, Florida, USA. Pineda_J@kids.wustl.edu

Country: United States

AGENCY: United States NINDS

GRANT: R01 NS052831

ACRONYM: NS

MEDLINETA: J Neurotrauma

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