Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity.

Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Research Abstract Details 

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Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Abstract Text:

K J MacBeth,Y T Ro,L Gehrke,J L Patterson,

The Leishmaniavirus capsid protein possesses an RNA endoribonuclease activity that cleaves viral positive-sense RNA at a specific, single site within the 5' untranslated region. The site of cleavage in LRV1-4 RNA was previously mapped to nucleotide 320 of the LRV1-4 genome. Here we show that an LRV2-1-derived substrate RNA transcript is also cleaved at a single site in an in vitro cleavage assay with LRV2-1 virions. Precise RNA cleavage site mapping in this divergent Old World virus, LRV2-1, confirms that cleavage is occurring within a region of homology to the LRV1 isolates. Substrate RNA transcripts possessing viral sequences from LRV1-4 or LRV2-1 genomes were assayed for susceptibility to cleavage by the cognate and noncognate capsid endoribonucleases to determine the level of substrate specificity.

Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Publishing Authors By Initials

KJ MacBeth,YT Ro,L Gehrke,JL Patterson,

For similar viruses: virion research abstracts see: viruses: virion research

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Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 122

Page Numbers: 193-200

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1997

Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. Information

Substance Name: Endoribonucleases

Registry Number: EC 3.1.-

Grant and Affiliation Information for Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity.

AFFILIATION: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

Country: JAPAN

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MEDLINETA: J Biochem

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