Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation.

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Abstract Text:

Takanori Aoki,Daisuke Sato,Yingyi Li,Takahisa Takino,Hisashi Miyamori,Hiroshi Sato,

Apolipoprotein E (apoE) in a human fetal brain cDNA library was identified, using the expression cloning method, as a gene product that formed a complex with latent matrix metalloproteinase (MMP)-2. Co-expression of membrane-type MMP-1 (MT1-MMP) with apoE in HEK293T cells reduced the amount of apoE secreted into the culture medium, whereas cell-associated apoE core protein was not affected. Incubation of native apoE protein with recombinant MT1-MMP resulted in the cleavage of apoE. Recombinant apoE protein fused to glutathione S-transferase (apoE-GST) was cleaved by MT1-MMP at the following peptide bonds; T(85)-M(86), K(93)-S(94), R(246)-L(247), A(255)-E(256) and G(296)-L(297). HT1080 cells transfected with the apoE gene, which express endogenous MT1-MMP, secreted a low level of apoE protein and its cleaved fragments, and treatment with MMP inhibitor BB94 induced accumulation of apoE and retardation of cell proliferation. Addition of apoE-GST protein to the culture of HEK293T cells suppressed cell proliferation, and stable transfection of the MT1-MMP gene partly abrogated the suppression. These results suggest that cleavage of apoE protein by MT1-MMP abrogates apoE-mediated suppression of cell proliferation.

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Publishing Authors By Initials

T Aoki,D Sato,Y Li,T Takino,H Miyamori,H Sato,

For similar thiophenes research abstracts see: thiophenes research

PUBMED ID PMID:

MEDLINE DATE:

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 137

Page Numbers: 95-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2005

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Keywords Mesh Terms:

KEYWORDS: Thiophenes

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation. Information

Substance Name: Metalloendopeptidases

Registry Number: EC 3.4.24.-

Grant and Affiliation Information for Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation.

AFFILIATION: Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-0934, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cleavage of apolipoprotein E by membrane-type matrix metalloproteinase-1 abrogates suppression of cell proliferation Related Publications

• Age related changes in the distribution of laryngeal glands in the human adult larynx.
• Anti-eosinophil peroxidase antibodies detected in patients with primary biliary cirrhosis.
• Assessment of bull fertility using a mucus penetration test and a human chorionic gonadotrophin stimulation test.
• Colonic mucosal lesions associated with long-term administration of non-steroidal anti-inflammatory drugs.
• Decreased expression levels of CD22 and L-selectin on peripheral blood B lymphocytes from patients with bullous pemphigoid.
• Differences in acetazolamide vasoreactivity in patients with acute and chronic occlusion of the internal carotid artery.
• Effect of low-molecular-weight heparin on the commitment of bone marrow cells to liver sinusoidal endothelial cells in CCl(4)-induced liver injury.
• Effects of gonadotropin releasing hormone and prostaglandin F(2)alpha on the reproductive performance in postpartum cows.
• Exacerbation of dietary steatohepatitis and fibrosis in obese, diabetic KK-A(y) mice.
• Expression of MUC1 and MUC2 mucin antigens in intrahepatic bile duct tumors: its relationship with a new morphological classification of cholangiocarcinoma.
• Expression of MUC1 and MUC2 mucins in gastric carcinomas: its relationship with the prognosis of the patients.
• Expression of mucin antigens in human cancers and its relationship with malignancy potential.
• Gene expression of gastric type mucin (MUC5AC) in pancreatic tumors: its relationship with the biological behavior of the tumor.
• Hepatic steatosis is a predictor of poor response to interferon alpha-2b and ribavirin combination therapy in Japanese patients with chronic hepatitis C.
• Immunohistochemical study of mucin carbohydrates and core proteins in human pancreatic tumors.
• Infective Endocarditis Associated with Sjogren's Syndrome.
• Isokinetic external rotation strength of shoulder : correlation with age and muscle size.
• MUC-1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas.
• MUC2 gene expression is found in noninvasive tumors but not in invasive tumors of the pancreas and liver: its close relationship with prognosis of the patients.
• Mechanism by which Bombyx mori hemocytes recognize microorganisms: direct and indirect recognition systems for PAMPs.
• Modeling of daily operation in proton radiotherapy by Monte Carlo method.
• Mucin antigen expression in gastric carcinomas of young and old adults.
• Prostaglandin E(2) levels in uterine tissues and its relationship with uterine and luteal progesterone during the estrous cycle in dairy cows.
• Reconstruction of scalp and cranium defect utilizing latissimus dorsi musculocutaneous and serratus anterior muscle free flaps with interpositional anastomosis of T-shaped flap artery: case report.
• Second messenger systems mediating sex pheromone and amino Acid sensitivity in goldfish olfactory receptor neurons.
• Study on the appearance of the cavity in the corpus luteum of cows by using ultrasonic scanning.
• The expression of several types of mucin is related to the biological behavior of pancreatic neoplasms.
• The role of leptin in progression of non-alcoholic fatty liver disease.
• Voglibose potentiates the hepatotoxicity of carbon tetrachloride and acetaminophen by inducing CYP2E1 in rats.
• [Development and operation of cultured cell experiment with TR-IA sounding rocket]