Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1.

Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1. Research Abstract Details 

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Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1. Abstract Text:

Shunsuke Kon,Kenji Tanabe,Toshio Watanabe,Hisataka Sabe,Masanobu Satake,

E-cadherin is a central component of the adherens junction in epithelial cells and continuously undergoes endocytosis via clathrin-coated vesicles and/or caveolae depending on the cell type. In this study, we examined the role of SMAP1, a clathrin-interacting GTPase-activating protein (GAP) for the ADP-ribosylation factor 6 (Arf6) GTPase, in E-cadherin endocytosis. Mardin-Darby canine kidney (MDCK) epithelial cells were used as a model, and SMAP1 localized in the cytoplasm and along the adherens junction where E-cadherin was present. Next, activity of SMAP1 was compared with that of other Arf6GAPs (and/or an effector of Arf6-GTP), namely GIT1 and AMAP2/DDEF2. Overexpression of SMAP1 but not GIT1 nor AMAP2/DDEF2 strongly inhibited basal, as well as phorbolester-induced, internalization of E-cadherin. Notably, AMAP2/DDEF2 rather enhanced the caveolae-mediated incorporation of a membrane protein other than E-cadherin. Thus, in MDCK cells, E-cadherin appeared to be endocytosed solely through SMAP1-regulated clathrin-coated vesicles. Furthermore, MDCK cells overexpressing SMAP1 showed a reduced degree of cell migration compared to untransfected cells, as assessed by wound healing and Transwell assays, and this reduction in migration appeared to be due to the accumulation of E-cadherin at the adherens junction in cells overexpressing SMAP1. Collectively, SMAP1 likely represents a key Arf6GAP in clathrin dependent endocytosis of E-cadherin in MDCK cells. This activity of SMAP1 in E-cadherin turnover may be involved in epithelial organization and/or epithelial-mesenchymal transition.

Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1. Publishing Authors By Initials

S Kon,K Tanabe,T Watanabe,H Sabe,M Satake,

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Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Experimental cell research

VOLUME: 314

Page Numbers: 1415-28

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ISSN: 0014-4827

DAY: 17

MONTH: 11

YEAR: 2007

Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1. Information

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LANGUAGE: eng

NlmUniqueID: 373226

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Country: United States

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MEDLINETA: Exp Cell Res

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