Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes.

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Abstract Text:

Hajime Bando,Karen G Shadrach,Mary E Rayborn,John W Crabb,Joe G Hollyfield,

Clathrin was identified in a recent proteomic analysis of Bruch's membrane from age-related macular degeneration (AMD) donor eyes. The present study was conducted to determine the localization of clathrin in AMD tissues and to compare this distribution and relative content with that in non-AMD control tissues. The distribution of adaptin, which is functionally linked to clathrin, was also evaluated. Human eyes were from donors between 66 and 94 years of age; 13 eyes were from donors with AMD and 13 from non-AMD donors. Bruch's membrane and choroid from the macula of each donor eye were prepared for immunohistochemistry and Western blotting. Differences in immunoreactivity were quantitated. Drusen, Bruch's membrane and choroid from AMD tissues showed greater immunoreactivity for clathrin and adaptin than did non-AMD tissues. Western blots also showed more intense clathrin and adaptin immunoreactivity in AMD tissues than were present in non-AMD samples. This study suggests that accumulation of clathrin and adaptin in drusen, Bruch's membrane and choroid may reflect a higher rate of clathrin mediated endocytosis in AMD tissues. Alternatively, the accumulation of these proteins in these extracellular compartments may reflect a higher susceptibility to oxidative damage.

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Publishing Authors By Initials

H Bando,KG Shadrach,ME Rayborn,JW Crabb,JG Hollyfield,

For similar pigment epithelium of eye research abstracts see: pigment epithelium of eye research

PUBMED ID PMID:

MEDLINE DATE:

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental eye research

VOLUME: 84

Page Numbers: 135-42

Journal Abbreviation: Exp. Eye Res.

ISSN: 0014-4835

DAY: 13

MONTH: 11

YEAR: 2006

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370707

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Keywords Mesh Terms:

KEYWORDS: Pigment Epithelium of Eye

MESH TERMS: metabolism

Chemical & Substance for Abstract: Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes. Information

Substance Name: Clathrin

Registry Number: 0

Grant and Affiliation Information for Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes.

AFFILIATION: Cole Eye Institute (i-31), Department of Ophthalmology, Cleveland Clinic Foundation Lerner College of Medicine of Case Western Reserve University, 9500 Euclid Avenue, Cleveland, OH 44195, USA.

Country: England

AGENCY: United States NEI

GRANT: EY014240

ACRONYM: EY

MEDLINETA: Exp Eye Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes Related Publications

• Angiotensin II and its receptor subtypes in the human retina.
• Annexins in Bruch's membrane and drusen.
• Clathrin and adaptin accumulation in drusen, Bruch's membrane and choroid in AMD and non-AMD donor eyes.
• Semenogelins in the human retina: Differences in distribution and content between AMD and normal donor tissues.
• The retinal pigment epithelium apical microvilli and retinal function.