Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose.

Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Research Abstract Details 

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Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Abstract Text:

Cabral, ,Roxana B Medina de Figueroa,Silvia N ,

Citrate metabolism by Enterococcus faecium ET C9 and Enterococcus durans Ov 421 was studied as sole energy source and in presence of glucose or lactose. Both strains utilized citrate as the sole energy source. Enterococcus faecium ET C9 showed diauxic growth in the presence of a limiting concentration of glucose. Neither strain used citrate until glucose was fully metabolized. The strains showed co-metabolism of citrate and lactose. Lactate, acetate, formate, and flavour compounds (diacetyl, acetoin, and 2,3-butanediol) were detected in both strains. The highest production of flavour compounds was detected during growth of E. durans Ov 421 in media supplemented with citrate-glucose and citrate-lactose. Citrate lyase was inducible in both strains. Acetate kinase activities presented the highest values in LAPTc medium, with E. faecium ET C9 displaying a specific activity 2.4-fold higher than E. durans. The highest levels of alpha-acetolactate synthase specific activity were detected in E. durans grown in LAPTc+g, in accordance with the maximum production of flavour compounds detected in this medium. Diacetyl and acetoinreductases displayed lower specific activity values in the presence of citrate. Enterococcus faecium and E. durans displayed citrate lyase, acetate kinase, alpha-acetolactate synthase, and diacetyl and acetoin reductase activities. These enzymes are necessary for conversion of citrate to flavour compounds that are important in fermented dairy products.

Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Publishing Authors By Initials

ME Cabral,MC ,RB Medina de Figueroa,SN ,

For similar fluids and secretions: bodily secretions: milk research abstracts see: fluids and secretions: bodily secretions: milk research

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Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Canadian journal of microbiology

VOLUME: 53

Page Numbers: 607-15

Journal Abbreviation: Can. J. Microbiol.

ISSN: 0008-4166

DAY: 30

MONTH: May

YEAR: 2007

Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Information

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LANGUAGE: eng

NlmUniqueID: 372707

Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Keywords Mesh Terms:

KEYWORDS: Milk

MESH TERMS: microbiology

Chemical & Substance for Abstract: Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose. Information

Substance Name: Lactose

Registry Number: 63-42-3

Grant and Affiliation Information for Citrate metabolism by Enterococcus faecium and Enterococcus durans isolated from goat's and ewe's milk: influence of glucose and lactose.

AFFILIATION: Planta Piloto de Procesos Industiales Microbiológicos (PROIMI), Av. Belgrano y Pasaje Caseros, 4000, Tucumán, Argentina.

Country: Canada

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MEDLINETA: Can J Microbiol

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