Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer.

Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer. Research Abstract Details 

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Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer. Abstract Text:

Laura Beaster-Jones,Michael Schubert,Linda Z Holland,Laura Beaster-Jones,Michael Schubert,Linda Z Holland,

To gain insights into the relation between evolution of cis-regulatory DNA and evolution of gene function, we identified tissue-specific enhancers of the engrailed gene of the basal chordate amphioxus (Branchiostoma floridae) and compared their ability to direct expression in both amphioxus and its nearest chordate relative, the tunicate Ciona intestinalis. In amphioxus embryos, the native engrailed gene is expressed in three domains - the eight most anterior somites, a few cells in the central nervous system (CNS) and a few ectodermal cells. In contrast, in C. intestinalis, in which muscle development is highly divergent, engrailed expression is limited to the CNS. To characterize the tissue-specific enhancers of amphioxus engrailed, we first showed that 7.8kb of upstream DNA of amphioxus engrailed directs expression to all three domains in amphioxus that express the native gene. We then identified the amphioxus engrailed muscle-specific enhancer as the 1.2kb region of upstream DNA with the highest sequence identity to the mouse en-2 jaw muscle enhancer. This amphioxus enhancer directed expression to both the somites in amphioxus and to the larval muscles in C. intestinalis. These results show that even though expression of the native engrailed has apparently been lost in developing C. intestinalis muscles, they express the transcription factors necessary to activate transcription from the amphioxus engrailed enhancer, suggesting that gene networks may not be completely disrupted if an individual component is lost.

Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer. Publishing Authors By Initials

L Beaster-Jones,M Schubert,LZ Holland,L Beaster-Jones,M Schubert,LZ Holland,

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Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Mechanisms of development

VOLUME: 124

Page Numbers: 532-42

Journal Abbreviation: Mech. Dev.

ISSN: 0925-4773

DAY: 12

MONTH: 06

YEAR: 2007

Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer. Information

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LANGUAGE: eng

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AFFILIATION: Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093-0202, USA.

Country: Ireland

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MEDLINETA: Mech Dev

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