CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin.

CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Research Abstract Details 

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CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Abstract Text:

Akira Kawata,Junko Iida,Mitsunobu Ikeda,Yuji Sato,Hiroki Mori,Ai Kansaku,Kazutaka Sumita,Naoyuki Fujiwara,Chiaki Rokukawa,Mamiko Hamano,Susumu Hirabayashi,Yutaka Hata,

Membrane-associated guanylate kinase inverted (MAGI)-1 plays a role as a scaffold at cell junctions in non-neuronal cells, while S-SCAM, its neuronal isoform, is involved in the organization of synapses. A search for MAGI-1-interacting proteins by yeast two-hybrid screening of a kidney cDNA library yielded dendrin. As dendrin was originally reported as a brain-specific postsynaptic protein, we tested the interaction between dendrin and S-SCAM and revealed that dendrin binds to the WW domains of S-SCAM. Dendrin is known to be dendritically translated but its function is largely unknown. To gain insights into the physiological meaning of the interaction, we performed a second yeast two-hybrid screening using dendrin as a bait. We identified CIN85, an endocytic scaffold protein, as a putative dendrin-interactor. Immunocytochemistry and subcellular fractionation analysis supported the synaptic localization of CIN85. The first SH3 domain and the C-terminal region of CIN85 bind to the proline-rich region and the N-terminal region of dendrin, respectively. In vitro experiments suggest that dendrin forms a ternary complex with CIN85 and S-SCAM and that this complex formation facilitates the recruitment of dendrin and S-SCAM to vesicle-like structures where CIN85 is accumulated.

CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Publishing Authors By Initials

A Kawata,J Iida,M Ikeda,Y Sato,H Mori,A Kansaku,K Sumita,N Fujiwara,C Rokukawa,M Hamano,S Hirabayashi,Y Hata,

For similar investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research abstracts see: investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research

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CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 931-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2006

CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Keywords Mesh Terms:

KEYWORDS: Two-Hybrid System Techniques

MESH TERMS: metabolism

Chemical & Substance for Abstract: CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Information

Substance Name: MAGI-1 protein, rat

Registry Number: EC 2.7.4.8

Grant and Affiliation Information for CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin.

AFFILIATION: Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8519.

Country: Japan

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MEDLINETA: J Biochem

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