Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart.

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Abstract Text:

S N Patiyal,S Sharma,

The aim of this study was to analyze the effects of chronic administration of the beta-adrenoceptor agonist clenbuterol (2 mg/kg body weight/day for a period of 30 days) on the major contractile protein (myosin) in the left ventricular muscle of the adult mouse heart. Separation of myosin heavy chain (MHC) isoforms on 7.5 % glycerol SDS-PAGE and subsequent quantification of the gels by laser densitometry showed a 6.5-fold increase in the beta-isoform of MHC in the clenbuterol-treated group. The alpha : beta ratio of these two isoforms in the control group was 98.16+/-0.14 %: 1.83+/-0.14 %, whereas in the treated group it was 88.05+/-1.15 % : 11.95+/-1.15 %. Actomyosin ATPase activity assay demonstrated a significant (20 %) decline in ATPase activity of the tissue in the beta-agonist-treated group. These results suggest that chronic clenbuterol treatment is capable to induced the transformation of MHC isoforms increasing the slow beta-MHC isoform, which may contribute to the altered contractile mechanics of clenbuterol-treated hearts.

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Publishing Authors By Initials

SN Patiyal,S Sharma,

For similar proteins: protein isoforms research abstracts see: proteins: protein isoforms research

PUBMED ID PMID:

MEDLINE DATE:

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Physiological research / Academia Scientiarum Bohe

VOLUME: 56

Page Numbers: 275-83

Journal Abbreviation: Physiol Res

ISSN: 0862-8408

DAY: 22

MONTH: 06

YEAR: 2006

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9112413

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Keywords Mesh Terms:

KEYWORDS: Protein Isoforms

MESH TERMS: metabolism

Chemical & Substance for Abstract: Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart. Information

Substance Name: Myosins

Registry Number: EC 3.6.1.4

Grant and Affiliation Information for Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart.

AFFILIATION: Department of Biosciences, Himachal Pradesh University sumer Hill, Shimla, India. snpatiyal@yahoo.co.in

Country: Czech Republic

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Physiol Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Chronic oral administration of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain MHC expression in adult mouse heart Related Publications

• Behavioral manifestations of sturge-weber syndrome: a case report.
• Body size and pelvic area relationships at puberty and two-month-pregnancy in buffalo - heifers.
• Calcium-modulated rod outer segment membrane guanylate cyclase type 1 transduction machinery in the testes.
• Ceramide synthase inhibition by fumonisin B1 treatment activates sphingolipid-metabolizing systems in mouse liver.
• Cerebellopointine angle medulloblastoma.
• Conserved F84 and P86 residues in alphaB-crystallin are essential to effectively prevent the aggregation of substrate proteins.
• Current status of rotational atherectomy.
• Decreased expressions of c-myc and H-ras oncogenes in vitamin E succinate induced morphologically differentiated murine B-16 melanoma cells in culture.
• Dens evaginatus of anterior teeth (talon cusp) associated with other odontogenic anomalies.
• Dystocia due to urinary bladder carcinoma in two water buffaloes (bubalus bubalis) - Clinical case report.
• Effect of vitamin E succinate and a cAMP-stimulating agent on the expression of c-myc and N-myc and H-ras in murine neuroblastoma cells.
• Exploring evolving concepts and challenges in forensic psychiatry.
• Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0.
• In vivo pelvimetry in buffaloes (bos bubalus ).
• Induction of estrus in anestrous cows treated with 'Fertivet'.
• Influence of body mass index and self-administration of hCG on the outcome of IVF cycles: a prospective cohort study.
• Insulin resistance and clinical aspects of non-alcoholic steatohepatitis (NASH).
• Interleukins and STAT signaling.
• Lysozyme refolding with cyclodextrins: structure-activity relationship.
• Metalloantimalarials: synthesis and characterization of a novel agent possessing activity against Plasmodium falciparum.
• Novel frequenin-modulated Ca2+-signaling membrane guanylate cyclase (ROS-GC) transduction pathway in bovine hippocampus.
• Pituitary abscess associated with prolactinoma.
• Right hemothorax from leaking aortocoronary vein graft giant aneurysm.
• S-adenosylmethionine or 5'-methylthioadenosine are unable to prevent fumonisin B1 hepatotoxicity in mice despite increased oxidation in liver.
• Single photon emission computed tomography and positron emission tomography imaging of multi-drug resistant P-glycoprotein--monitoring a transport activity important in cancer, blood-brain barrier function and Alzheimer's disease.
• Studies on follicular patterns in buffalo-heifers.
• Studies on lactic dehydrogenase and sorbitol dehydrogenase release in relation to deep freezing of buffalo semen in certain extenders.
• Studies on serum progesterone levels in relation to occurrence of uterine torsion in buffaloes (Bubalus bubalis ).
• Thyroglossal duct remnant: seven years analysis.
• Treatment resistance in unipolar depression: Is it an iatrogenic phenomenon caused by antidepressant treatment of patients with a bipolar diathesis?