Chronic ethanol exposure induces alterations in the nucleocytoplasmic transport in growing astrocytes.

Chronic ethanol exposure induces alterations in the nucleocytoplasmic transport in growing astrocytes. Research Abstract Details 

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Chronic ethanol exposure induces alterations in the nucleocytoplasmic transport in growing astrocytes. Abstract Text:

Nucleocytoplasmic transport is a crucial process for cell function. We assessed the general effect of chronic alcohol exposure on this transport in growing astrocytes for the first time. Import and export of proteins to the nucleus were examined by pulse-chase experiments using (3)H-methionine, and we showed that ethanol induces a delay in both processes. Furthermore, we took an approach to evaluate the mechanisms involved in this effect. Whereas alcohol did not affect the amount and the distribution of several representative proteins that participate in nuclear import, such as RanBP1, RanGAP1 and the importins alpha2 and beta3, it decreased the amount of Exp1/CRM1, which is a general export receptor involved in the nuclear export. In addition, the density and distribution of nuclear pore complexes, which contribute to nucleocytoplasmic transport, were also affected by ethanol. These effects can be related with changes found in the content of several proteins associated with the nuclear envelope and the nuclear pore complex structure such as lamins A/C, and nucleoporins p62 and RanBP2, respectively. These results suggest that ethanol could interfere with some of the important processes regulated by nucleocytoplasmic transport in astrocytes and support the idea that one of the main ethanol targets is intracellular transport.

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Chronic ethanol exposure induces alterations in the nucleocytoplasmic transport in growing astrocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neurochemistry

VOLUME: 106

Page Numbers: 1914-28

Journal Abbreviation: J. Neurochem.

ISSN: 1471-4159

DAY: 9

MONTH: 06

YEAR: 2008

Chronic ethanol exposure induces alterations in the nucleocytoplasmic transport in growing astrocytes. Information

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LANGUAGE: eng

NlmUniqueID: 2985190

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Grant and Affiliation Information for Chronic ethanol exposure induces alterations in the nucleocytoplasmic transport in growing astrocytes.

AFFILIATION: Section of Biología y Patología Celular, Centro de Investigación, Hospital 'La Fe', Valencia, Spain.

Country: England

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MEDLINETA: J Neurochem

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