Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.

Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus. Research Abstract Details 

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Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus. Abstract Text:

Fabio Caradonna,Daniele Bellavia,Ann Maria Clemente,Giorgia Sisino,Rainer Barbieri,Fabio Caradonna,Daniele Bellavia,Ann Maria Clemente,Giorgia Sisino,Rainer Barbieri,

In this paper the chromosomal localization and molecular cloning and characterization of three 5S rDNA clusters of 700 bp (base pairs), 900 bp, and 950 bp in the sea urchin Paracentrotus lividus are reported. Southern blot hybridization demonstrated the existence of three 5S rDNA repeats of differing length in the P. lividus genome. Fluorescence in situ hybridization analysis, performed in parallel on both haploid and diploid metaphases and interphase nuclei using different 5S rDNA units as probes, localized these 5S rDNA clusters in 3 different pairs of P. lividus chromosomes. This is the first complete gene mapping not only in a sea urchin but also in the phylum of echinoderms as a whole.

Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus. Publishing Authors By Initials

F Caradonna,D Bellavia,AM Clemente,G Sisino,R Barbieri,F Caradonna,D Bellavia,AM Clemente,G Sisino,R Barbieri,

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Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genome / National Research Council Canada = Génome

VOLUME: 50

Page Numbers: 867-70

Journal Abbreviation: Genome

ISSN: 0831-2796

DAY: 25

MONTH: Sep

YEAR: 2007

Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus. Information

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LANGUAGE: eng

NlmUniqueID: 8704544

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Grant and Affiliation Information for Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.

AFFILIATION: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Viale delle Scienze, 90128, Palermo, Italy.

Country: Canada

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MEDLINETA: Genome

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