Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes.

Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Research Abstract Details 

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Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Abstract Text:

Masao Takeuchi,Kikuko Takeuchi,Arihiro Kohara,Motonobu Satoh,Setsuko Shioda,Yutaka Ozawa,Azusa Ohtani,Keiko Morita,Takashi Hirano,Masanori Terai,Akihiro Umezawa,Hiroshi Mizusawa,

Human mesenchymal stem cells (hMSCs) are expected to be an enormous potential source for future cell therapy, because of their self-renewing divisions and also because of their multiple-lineage differentiation. The finite lifespan of these cells, however, is a hurdle for clinical application. Recently, several hMSC lines have been established by immortalized human telomerase reverse transcriptase gene (hTERT) alone or with hTERT in combination with human papillomavirus type 16 E6/E7 genes (E6/E7) and human proto-oncogene, Bmi-1, but have not so much been characterized their karyotypic stability in detail during extended lifespan under in vitro conditions. In this report, the cells immortalized with the hTERT gene alone exhibited little change in karyotype, whereas the cells immortalized with E6/E7 plus hTERT genes or Bmi-1, E6 plus hTERT genes were unstable regarding chromosome numbers, which altered markedly during prolonged culture. Interestingly, one unique chromosomal alteration was the preferential loss of chromosome 13 in three cell lines, observed by fluorescence in situ hybridization (FISH) and comparative-genomic hybridization (CGH) analysis. The four cell lines all maintained the ability to differentiate into both osteogenic and adipogenic lineages, and two cell lines underwent neuroblastic differentiation. Thus, our results were able to provide a step forward toward fulfilling the need for a sufficient number of cells for new therapeutic applications, and substantiate that these cell lines are a useful model for understanding the mechanisms of chromosomal instability and differentiation of hMSCs.

Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Publishing Authors By Initials

M Takeuchi,K Takeuchi,A Kohara,M Satoh,S Shioda,Y Ozawa,A Ohtani,K Morita,T Hirano,M Terai,A Umezawa,H Mizusawa,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: nucleotidyltransferases: dna nucleotidyltransferases: dna-directed dna polymerase: rna-directed dna polymerase: telomerase research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: nucleotidyltransferases: dna nucleotidyltransferases: dna-directed dna polymerase: rna-directed dna polymerase: telomerase research

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Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: In vitro cellular & developmental biology. Animal

VOLUME: 43

Page Numbers: 129-38

Journal Abbreviation: In Vitro Cell. Dev. Biol. Anim

ISSN: 1071-2690

DAY: 21

MONTH: 05

YEAR: 2007

Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Information

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LANGUAGE: eng

NlmUniqueID: 9418515

Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Keywords Mesh Terms:

KEYWORDS: Telomerase

MESH TERMS: genetics

Chemical & Substance for Abstract: Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes. Information

Substance Name: Telomerase

Registry Number: EC 2.7.7.49

Grant and Affiliation Information for Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes.

AFFILIATION: Division of Bioresources, National Institute of Biomedical Innovation, Osaka, Japan. takeuchim@nibio.go.jp

Country: United States

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MEDLINETA: In Vitro Cell Dev Biol Anim

REFSOURCE: In Vitro Cell Dev Biol Anim. 2007 Sep-Oc

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