Chromosomal His-tagging: an alternative approach to membrane protein purification.

Chromosomal His-tagging: an alternative approach to membrane protein purification. Research Abstract Details 

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Chromosomal His-tagging: an alternative approach to membrane protein purification. Abstract Text:

Laurent Mamelli,Luc Dedieu,Emmanuelle ,Michael E Konkel,Jean-Marie ,Jean-Michel Bolla,

Membrane proteins are of keen interest to structural biologists, as they are known to act as receptors, adhesins, sensors, transporters, and signal-transducers of living cells. During the past few decades, the efforts made to study the bacterial membrane proteins have been impaired by the problems encountered during the production and purification of native proteins. Herein we demonstrate that the Campylobacter jejuni CadF protein, which was isolated using a novel purification strategy, exhibits biological activity as evidenced by channel activity in lipid bilayers. CadF, an E. coli OmpA-like protein, facilitates the binding of C. jejuni to the extracellular matrix component, fibronectin.

Chromosomal His-tagging: an alternative approach to membrane protein purification. Publishing Authors By Initials

L Mamelli,L Dedieu,E ,ME Konkel,JM ,JM Bolla,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Chromosomal His-tagging: an alternative approach to membrane protein purification. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proteomics

VOLUME: 7

Page Numbers: 399-402

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 3

MONTH: Feb

YEAR: 2007

Chromosomal His-tagging: an alternative approach to membrane protein purification. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101092707

Chromosomal His-tagging: an alternative approach to membrane protein purification. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: metabolism

Chemical & Substance for Abstract: Chromosomal His-tagging: an alternative approach to membrane protein purification. Information

Substance Name: Histidine

Registry Number: 71-00-1

Grant and Affiliation Information for Chromosomal His-tagging: an alternative approach to membrane protein purification.

AFFILIATION: UMR-MD1, IFR 48, Faculté de Médecine, Université de la Méditerranée, Marseille Cedex, France.

Country: Germany

AGENCY: United States NIDDK

GRANT: DK58911

ACRONYM: DK

MEDLINETA: Proteomics

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