Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved.

Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Research Abstract Details 

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Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Abstract Text:

Paula Chaves,Mateus Crespo,Catarina Ribeiro, Laranjeira,A Dias Pereira,Alexandra Suspiro,Paula Cardoso,C Nobre ,Jorge Soares,

Barrett's esophagus is lined by columnar and goblets cells with gastric and intestinal characteristics. Despite the association between goblet elements and malignancy, it was not demonstrated that other columnar cells lineages are not related to neoplasia. Chromosomal abnormalities were described in metaplasia adjacent to Barrett's neoplasia, but it is unknown which metaplastic lineages are involved. This work assessed the frequency and the type of chromosomal abnormalities in Barrett's esophagus without neoplasia and performed the identification of the metaplastic cells carrying chromosomal gains. Barrett's esophagus biopsies were collected and processed for short-term cell culture and cytogenetic analysis. Combined immunofluorescence/fluorescence in situ hybridization was performed in cases exhibiting chromosomal gains by using antisera against intestinal (MUC2) and gastric (MUC5AC and MUC6) apomucins and chromosome pericentromeric alpha satellite DNA probes for the chromosomes involved. Each case was scored for the number of spots (0, 1, 2, >2) in 200 nonoverlapping nuclei. Columnar and goblet cells were separately assessed. Short-term cell cultures were achieved in 40/60 cases (67%). There were clonal abnormalities in 27/40 cases (68%) and tetraploid (4n) clones in 10/40 (25%). Structural alterations were detected in 14/40 (35%) with recurrent breakpoints at 1q21, 15q15 and 15q22. Numerical changes (trisomies 7 and 18 and loss of Y) occurred in 16/40 (40%). Gains of chromosomes 7 and 18 were more frequent in columnar than in goblet cells (9.8% vs 0.7% (P<0.05)) and (7.9 vs 1.9% (P<0.05)) respectively. These alterations were detected in cells exhibiting gastric as well as intestinal features and were more frequent in cells without apomucin production. Conclusions: (1) chromosomal instability is a common finding in Barrett's esophagus without neoplasia. (2) The two metaplastic populations are committed, chromosomal gains being more frequent in columnar nongoblet than in goblet cells. (3) The two metaplastic phenotypes, gastric and intestinal, are equally involved.

Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Publishing Authors By Initials

P Chaves,M Crespo,C Ribeiro,C Laranjeira,AD Pereira,A Suspiro,P Cardoso,CN ,J Soares,

For similar mucoproteins: mucins research abstracts see: mucoproteins: mucins research

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Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Modern pathology : an official journal of the Unit

VOLUME: 20

Page Numbers: 788-96

Journal Abbreviation: Mod. Pathol.

ISSN: 0893-3952

DAY: 25

MONTH: 05

YEAR: 2007

Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Information

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LANGUAGE: eng

NlmUniqueID: 8806605

Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Keywords Mesh Terms:

KEYWORDS: Mucins

MESH TERMS: genetics

Chemical & Substance for Abstract: Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved. Information

Substance Name: mucin 6

Registry Number: 0

Grant and Affiliation Information for Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved.

AFFILIATION: Grupo de Estudo do Esófago de Barrett, Instituto Português de Oncologia de Lisboa de Francisco Gentil, EPE, Lisboa, Portugal. pchaves@ipolisboa.min-saude.pt

Country: United States

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MEDLINETA: Mod Pathol

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