Chromatography of living cells using supermacroporous hydrogels, cryogels.

Chromatography of living cells using supermacroporous hydrogels, cryogels. Research Abstract Details 

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Chromatography of living cells using supermacroporous hydrogels, cryogels. Abstract Text:

Maria B Dainiak,Igor Yu Galaev,Ashok Kumar,Fatima M Plieva,Bo Mattiasson,Maria B Dainiak,Igor Yu Galaev,Ashok Kumar,Fatima M Plieva,Bo Mattiasson,Maria B Dainiak,Igor Yu Galaev,Ashok Kumar,Fatima M Plieva,Bo Mattiasson,Maria B Dainiak,Igor Yu Galaev,Ashok Kumar,Fatima M Plieva,Bo Mattiasson,

The preparative cell separation is an intrinsic requirement of various diagnostic, biotechnological and biomedical applications. Affinity chromatography is a promising technique for cell separation and is based on the interaction between a cell surface receptor and an immobilised ligand. Most of the currently available matrices have pore size smaller than the size of the cells and are not suitable for cell chromatography due to column clogging. Another problem encountered in chromatographic separation of cells is a difficulty to elute bound cells from affinity surfaces. Application of novel adsorbents, supermacroporous monolithic cryogels, allows overcoming these problems. Cryogels are characterised by highly interconnected large (10-100 mum) pores, sponge-like morphology and high elasticity. They are easily derivatised with any ligand of choice. Convective migration can be used to transport the cells through the matrix. Target cells bind to affinity ligands, while other cells pass through the cryogel column non-retained and are removed during a washing step. Because of the spongy and elastic nature of the cryogel matrices, the cells are efficiently desorbed by mechanical compression of cryogels, which provides high cell viability and yields. The release of affinity bound cells by mechanical compression of a cryogel monolithic adsorbent is a unique and efficient way of cell detachment. This detachment strategy and the continuous macroporous structure make cryogels very attractive for application in cell separation chromatography.

Chromatography of living cells using supermacroporous hydrogels, cryogels. Publishing Authors By Initials

MB Dainiak,IY Galaev,A Kumar,FM Plieva,B Mattiasson,MB Dainiak,IY Galaev,A Kumar,FM Plieva,B Mattiasson,MB Dainiak,IY Galaev,A Kumar,FM Plieva,B Mattiasson,MB Dainiak,IY Galaev,A Kumar,FM Plieva,B Mattiasson,

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Chromatography of living cells using supermacroporous hydrogels, cryogels. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Advances in biochemical engineering/biotechnology

VOLUME: 106

Page Numbers: 101-27

Journal Abbreviation: Adv. Biochem. Eng. Biotechnol.

ISSN: 0724-6145

DAY: 9

MONTH: 10

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 8307733

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Grant and Affiliation Information for Chromatography of living cells using supermacroporous hydrogels, cryogels.

AFFILIATION: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 22100, Lund, Sweden, Bo.Mattiasson@biotek.lu.se.

Country: Germany

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MEDLINETA: Adv Biochem Eng Biotechnol

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