Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells.

Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Research Abstract Details 

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Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Abstract Text:

Ryosuke Obi,Michihisa Tohda,Qi Zhao,Nobuko Obi,Hitomi Hori,Yukihisa Murakami,Hirozo Goto,Yutaka Shimada,Hiroshi Ochiai,Kinzo Matsumoto,Ryosuke Obi,Michihisa Tohda,Qi Zhao,Nobuko Obi,Hitomi Hori,Yukihisa Murakami,Hirozo Goto,Yutaka Shimada,Hiroshi Ochiai,Kinzo Matsumoto,Ryosuke Obi,Michihisa Tohda,Qi Zhao,Nobuko Obi,Hitomi Hori,Yukihisa Murakami,Hirozo Goto,Yutaka Shimada,Hiroshi Ochiai,Kinzo Matsumoto,

Macrophage colony stimulating factor (M-CSF) is a cytokine which has been recently reported to have a neuroprotective effect on ischemic rat brain. In this study, we investigated the effect of chotosan, an oriental medicine, which has been clinically demonstrated to be effective for the treatment of vascular dementia, on M-CSF gene expression in rats with permanent occlusion of bilateral common carotid arteries (P2VO) in vivo and in a C6Bu-1 glioma cell line in vitro. The expression level of M-CSF mRNA in the cerebral cortices of P2VO rats was significantly higher than that in the cerebral cortices of sham-operated animals. Repeated treatment of P2VO rats with chotosan (75 mg/kg per day) for 4 d after P2VO significantly increased the expression level of M-CSF mRNA in the cortex but it had no effect on the expression of beta-actin, granulocyte colony stimulating factor (G-CSF), granulocyte/macrophage colony stimulating factor (GM-CSF) mRNAs. Moreover, the present in vitro studies revealed that chotosan treatment (10-100 mug/ml) of C6Bu-1 glioma cells dose-dependently enhanced M-CSF mRNA expression without affecting the expression of G-CSF, GM-CSF, and inducible nitric oxide synthase mRNAs. The effect of chotosan was reversed by Ro 31-8220 (1 muM), a selective protein kinase C (PKC) inhibitor, but not by H-89 (10 muM), a selective protein kinase A (PKA) inhibitor. These findings suggest that the upregulatory effect of chotosan on M-CSF mRNA expression involves PKC and may play an important role in the anti-vascular dementia action of this formula.

Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Publishing Authors By Initials

R Obi,M Tohda,Q Zhao,N Obi,H Hori,Y Murakami,H Goto,Y Shimada,H Ochiai,K Matsumoto,R Obi,M Tohda,Q Zhao,N Obi,H Hori,Y Murakami,H Goto,Y Shimada,H Ochiai,K Matsumoto,R Obi,M Tohda,Q Zhao,N Obi,H Hori,Y Murakami,H Goto,Y Shimada,H Ochiai,K Matsumoto,

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Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biological & pharmaceutical bulletin

VOLUME: 30

Page Numbers: 2250-6

Journal Abbreviation: Biol. Pharm. Bull.

ISSN: 0918-6158

DAY: 2

MONTH: Dec

YEAR: 2007

Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Information

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LANGUAGE: eng

NlmUniqueID: 9311984

Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Keywords Mesh Terms:

KEYWORDS: Thiazoles

MESH TERMS: physiology

Chemical & Substance for Abstract: Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells. Information

Substance Name: Protein Kinase C

Registry Number: EC 2.7.11.13

Grant and Affiliation Information for Chotosan enhances macrophage colony-stimulating factor mRNA expression in the ischemic rat brain and C6Bu-1 glioma cells.

AFFILIATION: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.

Country: Japan

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MEDLINETA: Biol Pharm Bull

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