Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling.

Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Research Abstract Details 

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Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Abstract Text:

Catherine R Wasser,Mert Ertunc,Xinran Liu,Ege T Kavalali,

Cholesterol is a prominent component of nerve terminals. To examine cholesterol's role in central neurotransmission, we treated hippocampal cultures with methyl-beta-cyclodextrin, which reversibly binds cholesterol, or mevastatin, an inhibitor of cholesterol biosynthesis, to deplete cholesterol. We also used hippocampal cultures from Niemann-Pick type C1-deficient mice defective in intracellular cholesterol trafficking. These conditions revealed an augmentation in spontaneous neurotransmission detected electrically and an increase in spontaneous vesicle endocytosis judged by horseradish peroxidase uptake after cholesterol depletion by methyl-beta-cyclodextrin. In contrast, responses evoked by action potentials and hypertonicity were severely impaired after the same treatments. The increase in spontaneous vesicle recycling and the decrease in evoked neurotransmission were reversible upon cholesterol addition. Cholesterol removal did not impact on the low level of evoked neurotransmission seen in the absence of synaptic vesicle SNARE protein synaptobrevin-2 whereas the increase in spontaneous fusion remained. These results suggest that synaptic cholesterol balances evoked and spontaneous neurotransmission by hindering spontaneous synaptic vesicle turnover and sustaining evoked exo-endocytosis.

Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Publishing Authors By Initials

CR Wasser,M Ertunc,X Liu,ET Kavalali,

For similar polycyclic compounds: macrocyclic compounds: cyclodextrins: beta-cyclodextrins research abstracts see: polycyclic compounds: macrocyclic compounds: cyclodextrins: beta-cyclodextrins research

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Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of physiology

VOLUME: 579

Page Numbers: 413-29

Journal Abbreviation: J. Physiol. (Lond.)

ISSN: 0022-3751

DAY: 14

MONTH: 12

YEAR: 2006

Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Information

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LANGUAGE: eng

NlmUniqueID: 266262

Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Keywords Mesh Terms:

KEYWORDS: beta-Cyclodextrins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling. Information

Substance Name: Lovastatin

Registry Number: 75330-75-5

Grant and Affiliation Information for Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling.

AFFILIATION: Center for Basic Neuroscience, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9111, USA.

Country: England

AGENCY: United States NIMH

GRANT: MH068437

ACRONYM: MH

MEDLINETA: J Physiol

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