Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400.

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Abstract Text:

Paula ,Loreine ,Marcela ,Michael Seeger,

Aerobic bacteria, such as Burkholderia xenovorans LB400, are able to degrade a wide range of polychlorobiphenyls (PCBs). Generally, these bacteria are not able to transform chlorobenzoates (CBAs), which accumulate during PCB degradation. In this study, the effects of CBAs on the growth, the morphology and the proteome of Burkholderia xenovorans LB400 were analysed. 4-CBA and 2-CBA were observed to inhibit the growth of strain LB400 on glucose. Strain LB400 exposed to 4-CBA exhibited increased number and size of electron-dense granules in the cytoplasm, which could be polyphosphates. Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to characterise the molecular response of strain LB400 to 4-CBA. This compound induced the enzymes BenD and CatA of benzoate and catechol catabolic pathways. The induction of molecular chaperones DnaK and HtpG by 4-CBA indicated that the exposure to this compound constitutes a stressful condition for this bacterium. Additionally, the induction of some Krebs cycle enzymes was observed, probably as response to cellular energy requirements. This study contributes to the knowledge on the effects of CBA on the PCB-degrader Burkholderia xenovorans LB400.

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Publishing Authors By Initials

P ,L ,M ,M Seeger,

For similar investigative techniques: genetic techniques: sequence analysis: sequence analysis, protein research abstracts see: investigative techniques: genetic techniques: sequence analysis: sequence analysis, protein research

PUBMED ID PMID:

MEDLINE DATE:

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Archives of microbiology

VOLUME: 188

Page Numbers: 289-97

Journal Abbreviation: Arch. Microbiol.

ISSN: 0302-8933

DAY: 24

MONTH: 05

YEAR: 2007

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 410427

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Keywords Mesh Terms:

KEYWORDS: Sequence Analysis, Protein

MESH TERMS: metabolism

Chemical & Substance for Abstract: Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400.

AFFILIATION: Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química, Millennium Nucleus of Microbial Ecology, Universidad Técnica Federico Santa María, Av. España 1680, Valparaíso, Chile. michael.seeger@usm.cl

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Arch Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400 Related Publications

• ALS conformed use of echocardiography or ultrasound in resuscitation management.
• Alveolar fluid clearance in acute lung injury: what have we learned from animal models and clinical studies?
• Bacterial metabolism of polychlorinated biphenyls.
• Breast cancer: are estrogen metabolites carcinogenic?
• Chemotherapy of tumors in rats with certain ethylenephosphoramides.
• Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400.
• Combination of hormone therapy with sartan: an option for hypertensive postmenopausal women?
• Differential effects of mutations in NS4B on West Nile virus replication and inhibition of interferon signaling.
• Disseminated coccidioidomycosis complicated by vasculitis: a cause of fatal subarachnoid hemorrhage in two cases.
• Effect of aprotinin on renal dysfunction.
• Expression of late cell cycle genes and an increased proliferative capacity characterize very early relapse of childhood acute lymphoblastic leukemia.
• Fish oil in critical illness.
• Functionalized silicone nanofilaments: a novel material for selective protein enrichment.
• Gradient-recalled echo sequences in direct shoulder MR arthrography for evaluating the labrum.
• Hemichorea-hemiballism associated with an ipsilateral intraventricular cyst after resection of a meningioma.
• Highly efficient optical detection of surface-generated fluorescence.
• Inhibition studies of some phosphoramides against sarcoma 180.
• Instruction in image processing for residents in diagnostic radiology.
• Long-term treatment with sildenafil in chronic thromboembolic pulmonary hypertension.
• Low-dose continuous combinations of hormone therapy and biochemical surrogate markers for vascular tone and inflammation: transdermal versus oral application.
• MR imaging of joints: analytic optimization of GRE techniques at 1.5 T.
• Midterm outcomes of femoral arteries after percutaneous endovascular aortic repair using the Preclose technique.
• Normal variations in iliac venous anatomy.
• Spring ligament of the ankle: normal MR anatomy.
• The World Health Organization defines hormone replacement therapy as carcinogenic: Is this plausible?
• The absorption spectra of the aminopyridines.
• The risk of coronary heart disease in type 2 diabetic patients exposed to thiazolidinediones compared to metformin and sulfonylurea therapy.
• [In Process Citation]
• [Obstructive sleep apnoea and metabolic syndrome]
• [Problems in the assessment of driving capability in family practice]