Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector.

Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Research Abstract Details 

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Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Abstract Text:

Thomas Hofer,Wisit Tangkeangsirisin,Michael G Kennedy,Rose G Mage,Stephen J Raiker,Karthik Venkatesh,Hakjoo Lee,Roman J Giger,Christoph Rader,

NgR1, NgR2, and NgR3 which constitute the Nogo-66 receptor family are primarily expressed by neurons in the central nervous system (CNS) and believed to limit axonal growth and sprouting following CNS injury. In an attempt to define the expression and decipher the function of individual members of the Nogo-66 receptor family, we previously reported the generation of selective rabbit polyclonal antibodies. Here we exploit the same immune repertoires by phage display technology to generate rabbit monoclonal antibodies (mAbs) with nanomolar affinity to epitopes that are specific for NgR1 and NgR2, respectively, but at the same time conserved between mouse, rat, and human orthologs. Employing phage display vector pC3C, a newly designed phagemid optimized for the generation and selection of Fab libraries with human constant domains, rabbit mAbs were selected from chimeric rabbit/human Fab libraries, characterized in terms of specificity, affinity, and amino acid sequence, and finally converted to chimeric rabbit/human IgG. Using immunofluorescence microscopy and immunoprecipitation, we demonstrate strong and specific recognition of cell surface bound Nogo-66 receptor family members by chimeric rabbit/human IgG. The rabbit mAbs reported here together with their amino acid sequences constitute a defined panel of species cross-reactive reagents in infinite supply which will aid investigations toward a functional role of the Nogo-66 receptor family in and beyond the CNS.

Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Publishing Authors By Initials

T Hofer,W Tangkeangsirisin,MG Kennedy,RG Mage,SJ Raiker,K Venkatesh,H Lee,RJ Giger,C Rader,

For similar therapeutics: biological therapy: immunotherapy: immunization: immunotherapy, active: vaccination research abstracts see: therapeutics: biological therapy: immunotherapy: immunization: immunotherapy, active: vaccination research

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MEDLINE DATE:

Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunological methods

VOLUME: 318

Page Numbers: 75-87

Journal Abbreviation: J. Immunol. Methods

ISSN: 0022-1759

DAY: 7

MONTH: 11

YEAR: 2006

Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1305440

Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Keywords Mesh Terms:

KEYWORDS: Vaccination

MESH TERMS: immunology

Chemical & Substance for Abstract: Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. Information

Substance Name: Rtn4r protein, rat

Registry Number: 0

Grant and Affiliation Information for Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector.

AFFILIATION: Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1203, USA.

Country: Netherlands

AGENCY: United States NIAID

GRANT: Z01 AI000226-24

ACRONYM: AI

MEDLINETA: J Immunol Methods

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ACCESSION NUMBER: DQ648596

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