Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site.

Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Research Abstract Details 

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Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Abstract Text:

Wen-Jing Zhu,Miao Li,Xiao-Yun Wang,Wen-Jing Zhu,Miao Li,Xiao-Yun Wang,

Chemical modification was used to elucidate the essential amino acids in the catalytic activity of arginine kinase (AK) from Migratoria manilensis. Among six cysteine (Cys) residues only one Cys residue was determined to be essential in the active site by Tsou's method. Furthermore, the AK modified by DTNB can be fully reactivated by dithiothreitol (DTT) in a monophasic kinetic course. At the same time, this reactivation can be slowed down in the presence of ATP, suggesting that the essential Cys is located near the ATP binding site. The ionizing groups at the AK active site were studied and the standard dissociation enthalpy (DeltaH degrees ) was 12.38kcal/mol, showing that the dissociation group may be the guanidino of arginine (Arg). Using the specific chemical modifier phenylglyoxal (PG) demonstrated that only one Arg, located near the ATP binding site, is essential for the activity of AK.

Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Publishing Authors By Initials

WJ Zhu,M Li,XY Wang,WJ Zhu,M Li,XY Wang,

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Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: International journal of biological macromolecules

VOLUME: 41

Page Numbers: 564-71

Journal Abbreviation: Int. J. Biol. Macromol.

ISSN: 0141-8130

DAY: 21

MONTH: 07

YEAR: 2007

Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site.

AFFILIATION: College of Life Science, Shandong Agricultural University, Shandong Taian 271018, People's Republic of China.

Country: England

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MEDLINETA: Int J Biol Macromol

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