Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin.

Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Research Abstract Details 

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Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Abstract Text:

R Masaki,K Wada,H Matsubara,

The acetylation of spinach ferredoxin by acetic anhydride modified about four moles of amino groups. The absorption spectra, CD spectra, the fluorescence of sole tryptophan residue and the biological activity of acetylated ferredoxin were investigated. An equilibrium existed between two different states, D- and N-form, of the acetylated ferredoxin and was dependent on the cation concentration. D-form completely reverted to N-form upon the binding of one mole of cation, Na+ or Mg2+. Although the N-form was indistinguishable from native ferredoxin in every property tested, the D-form was significantly different from the N-form or native ferredoxin and was very unstable, especially at low salt concentrations. It is suggested that the amino groups might be important in maintaining the protein conformation by forming salt linkages, but may not be essential for the activity. Furthermore, since the D-form, unlike the N-form and native ferredoxin, was inactive in the ferredoxin-NADP+ reductase [EC 1.6.7.1] assay system and had no inhibitory effect in this system, it was considered to be incapable of forming a complex with ferredoxin-NADP+ reductase. On the other hand, the N-form of the modified ferredoxin was as active as native ferredoxin. It is suggested that amino groups of spinach ferredoxin are not essential for the redox reaction of ferredoxin or for complex formation with the reductase.

Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Publishing Authors By Initials

R Masaki,K Wada,H Matsubara,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 1-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1977

Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin. Information

Substance Name: Ferredoxin-NADP Reductase

Registry Number: EC 1.18.1.2

Grant and Affiliation Information for Chemical modification of spinach ferredoxin. Properties of acetylated spinach ferredoxin.

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Country: JAPAN

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MEDLINETA: J Biochem

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