Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate.

Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Research Abstract Details 

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Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Abstract Text:

T Sekiguchi,S Oshiro,E M Goingo,Y Nosoh,

The activity of glutamine synthetase [EC 6.3.2.1] from Bacillus stearothermophilus decreased slightly on modification with ethyl acetimidate. Acetamidination of 25--26 of the 2 epsilon-amino groups/subunit of the enzyme affected the maximum velocity, but not the Michaelis constant. The thermostability of the enzyme was considerably increased on acetamidination. Acetamidination of the enzyme did not affect the circular dichroism, the tryptophan fluorescence or the quenching effects of KI and acrylamide on the tryptophan emission. The fluorescence spectrum of p-toluidinylnaphthalene sulfonate bound to the enzyme changed on acetamidination.

Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Publishing Authors By Initials

T Sekiguchi,S Oshiro,EM Goingo,Y Nosoh,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

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Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 85

Page Numbers: 75-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1979

Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Chemical modification of epsilon-amino groups in glutamine synthetase from Bacillus stearothermophilus with ethyl acetimidate. Information

Substance Name: Glutamate-Ammonia Ligase

Registry Number: EC 6.3.1.2

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Country: JAPAN

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MEDLINETA: J Biochem

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