Chemical evolution as a tool for molecular discovery.

Chemical evolution as a tool for molecular discovery. Research Abstract Details 

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Chemical evolution as a tool for molecular discovery. Abstract Text:

S Jarrett Wrenn,Pehr B Harbury,

In modern academic and industrial laboratories, evolutionary strategies are used routinely to identify biopolymers with novel activities. Large libraries of nucleic acids (approximately 10(15)) or peptides and proteins (approximately 10(13)) can be subjected to multiple rounds of selective pressure, amplification, and diversification, yielding individual sequences with desirable properties. Although the evolutionary approach is a powerful search tool, the chemical nature of biopolymers is not suited for all purposes. Application of evolutionary strategies to libraries of arbitrary chemical composition would overcome this problem, and radically change traditional small-molecule discovery. The chemical make-up of in vitro evolution libraries has necessarily been limited, because library synthesis relies on enzymes. A great deal of current research focuses on expanding the chemical repertoire of in vitro evolution by (a) broadening enzyme substrate specificities to include unnatural building blocks, or (b) developing methods to translate DNA sequences into multistep organic syntheses. We discuss the strengths and weaknesses of the approaches, review the successes, and consider the future of chemical evolution as a tool.

Chemical evolution as a tool for molecular discovery. Publishing Authors By Initials

SJ Wrenn,PB Harbury,

For similar peptides research abstracts see: peptides research

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Chemical evolution as a tool for molecular discovery. Journal Published:

PUBLICATION TYPE: Review

Journal: Annual review of biochemistry

VOLUME: 76

Page Numbers: 331-49

Journal Abbreviation: Annu. Rev. Biochem.

ISSN: 0066-4154

DAY: 3

MONTH: 12

YEAR: 2007

Chemical evolution as a tool for molecular discovery. Information

Number of References: 83

LANGUAGE: eng

NlmUniqueID: 2985150

Chemical evolution as a tool for molecular discovery. Keywords Mesh Terms:

KEYWORDS: Peptides

MESH TERMS: metabolism

Chemical & Substance for Abstract: Chemical evolution as a tool for molecular discovery. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Chemical evolution as a tool for molecular discovery.

AFFILIATION: Department of Biochemistry, Stanford University, Stanford, California 94305, USA. sjwrenn@stanford.edu

Country: United States

AGENCY: United States NCCDPHP

GRANT: 1 DP1 0D000429

ACRONYM: DP

MEDLINETA: Annu Rev Biochem

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