Checkerboard DNA-DNA hybridization technology using digoxigenin detection.

Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Research Abstract Details 

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Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Abstract Text:

Lisa S Gellen,Glenn M Wall-Manning,Chris H Sissons,

Checkerboard DNA-DNA hybridization (CKB) is a technique that provides a simultaneous quantitative analysis of 40 microbial species against up to 28 mixed microbiota samples on a single membrane; using digoxigenin (DIG)-labeled, whole-genome DNA probes. Developed initially to study the predominantly gram-negative dental plaque microorganisms involved in periodontitis, we modified the probe species composition to focus on putative pathogens involved in the development of dental caries. CKB analysis is applicable to species from other biodiverse ecosystems and to a large number of samples. The major limitations are that high-quality DNA is required for the preparation of DIG-labeled probes and standards, and that probe specificity requires careful evaluation. Overall, CKB analysis provides a powerful ecological fingerprint of highly biodiverse microbiota based on key cultivable bacteria.

Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Publishing Authors By Initials

LS Gellen,GM Wall-Manning,CH Sissons,

For similar natural sciences: weights and measures: reference standards research abstracts see: natural sciences: weights and measures: reference standards research

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Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 353

Page Numbers: 39-67

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Keywords Mesh Terms:

KEYWORDS: Reference Standards

MESH TERMS: microbiology

Chemical & Substance for Abstract: Checkerboard DNA-DNA hybridization technology using digoxigenin detection. Information

Substance Name: Digoxigenin

Registry Number: 1672-46-4

Grant and Affiliation Information for Checkerboard DNA-DNA hybridization technology using digoxigenin detection.

AFFILIATION: Department of Pathology and Molecular Medicine, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand.

Country: United States

AGENCY: United States NIDCR

GRANT: DE 1272

ACRONYM: DE

MEDLINETA: Methods Mol Biol

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