Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings.

Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Research Abstract Details 

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Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Abstract Text:

Maximillian H Bailor,Catherine Musselman,Alexandar L Hansen,Kush Gulati,Dinshaw J Patel,Hashim M Al-Hashimi,Maximillian H Bailor,Catherine Musselman,Alexandar L Hansen,Kush Gulati,Dinshaw J Patel,Hashim M Al-Hashimi,

We present a protocol for determining the relative orientation and dynamics of A-form helices in 13C/15N isotopically enriched RNA samples using NMR residual dipolar couplings (RDCs). Non-terminal Watson-Crick base pairs in helical stems are experimentally identified using NOE and trans-hydrogen bond connectivity and modeled using the idealized A-form helix geometry. RDCs measured in the partially aligned RNA are used to compute order tensors describing average alignment of each helix relative to the applied magnetic field. The order tensors are translated into Euler angles defining the average relative orientation of helices and order parameters describing the amplitude and asymmetry of interhelix motions. The protocol does not require complete resonance assignments and therefore can be implemented rapidly to RNAs much larger than those for which complete high-resolution NMR structure determination is feasible. The protocol is particularly valuable for exploring adaptive changes in RNA conformation that occur in response to biologically relevant signals. Following resonance assignments, the procedure is expected to take no more than 2 weeks of acquisition and data analysis time.

Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Publishing Authors By Initials

MH Bailor,C Musselman,AL Hansen,K Gulati,DJ Patel,HM Al-Hashimi,MH Bailor,C Musselman,AL Hansen,K Gulati,DJ Patel,HM Al-Hashimi,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

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Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 2

Page Numbers: 1536-46

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: chemistry

Chemical & Substance for Abstract: Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings.

AFFILIATION: Department of Chemistry & Biophysics Research Division, The University of Michigan, Ann Arbor, Michigan 48109, USA.

Country: England

AGENCY: United States NIAID

GRANT: R01 AI066975-01

ACRONYM: AI

MEDLINETA: Nat Protoc

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