Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance.

Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Research Abstract Details 

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Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Abstract Text:

Jinli Wang,Renji Lv,Jingjuan Xu,Danke Xu,Hongyuan Chen,Jinli Wang,Renji Lv,Jingjuan Xu,Danke Xu,Hongyuan Chen,Jinli Wang,Renji Lv,Jingjuan Xu,Danke Xu,Hongyuan Chen,

Human immunoglobulin E (hIgE) is such an important protein, because of its involvement in allergic disease, that it is of significance to study the interactions between it and its recognizing elements. In this report an analytical strategy based on surface plasmon resonance (SPR) was developed to probe the pattern of interaction between hIgE and its recognizing molecules, including aptamers and antibodies. The affinity constants of hIgE for the antibody and the aptamer were compared first; the aptamer has more affinity than the antibody for human IgE. To study their pattern of interaction, three different binding approaches, including adding the antibody and the streptavidin-coupled aptamer to the sensing surface, were designed. The results showed that hIgE captured on the sensing surface could form a multivalent complex with the aptamer. An ELISA-like assay using the aptamer as both capture and detection probes was then developed. This work highlights an SPR method for characterizing the interaction between the protein and aptamers that is useful for study of biomolecular interaction patterns and binding properties. Figure Schematic diagram of the use of surface plasmon resonance for detection of the pattern of interaction of human IgE with its DNA aptamer and antibody.

Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Publishing Authors By Initials

J Wang,R Lv,J Xu,D Xu,H Chen,J Wang,R Lv,J Xu,D Xu,H Chen,J Wang,R Lv,J Xu,D Xu,H Chen,

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Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical and bioanalytical chemistry

VOLUME: 390

Page Numbers: 1059-65

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ISSN: 1618-2650

DAY: 15

MONTH: 12

YEAR: 2007

Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance.

AFFILIATION: School of Chemistry and Chemical Engineering, Key Lab of Analytical Chemistry for Life Science (MOE), Nanjing University, 22 Hankou Road, Nanjing, 210093, China.

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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