Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides.

Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Research Abstract Details 

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Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Abstract Text:

Y Takahashi,T Shirai,S Ishii,

Previously an enzyme, named acylagmatine amidohydrolase, hydrolyzing bleomycin B2 to bleomycinic acid and agmatine was found in the mycelia of Fusarium anguioides Sherbakoff. In this work the enzyme was purified further, but not completely. The crude enzyme preparation hydrolyzed various acylagmatines and also peptidyl arginine, but the latter activity could be separated from acylagmatine amidohydrolase activity by gel filtration on Sephadex G-100. The enzyme was inhibited by PCMB and its molecular weight was estimated as 65,000 by gel filtration. It showed substrate specificity with respect to the alkyl-chain length of the amine moiety. The other hydrolase fraction with activity toward Bz-Gly-Arg was found to be of a sort of carboxypeptidase, which preferentially hydrolyzed peptides with arginine or lysine at the carboxyl terminus, including bradykinin, but liberated neutral amino acids as well from the terminus when the penultimate residue of the substrates was phenylalanine. With Bz-Gly-Arg as substrate Fusarium carboxypeptidase was sensitive to chelating agents but not to diisopropyfluorophosphate, and its molecular weight was estimated to be 145,000.

Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Publishing Authors By Initials

Y Takahashi,T Shirai,S Ishii,

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Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 77

Page Numbers: 823-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1975

Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides. Information

Substance Name: Amidohydrolases

Registry Number: EC 3.5.-

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Country: JAPAN

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MEDLINETA: J Biochem

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