Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture.

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Abstract Text:

Emiko Aiba-Kojima,Nelson H Tsuno,Keita Inoue,Daisuke Matsumoto,Tomokuni Shigeura,Takahiro Sato,Hirotaka Suga,Harunosuke Kato,Takashi Nagase,Koichi Gonda,Isao Koshima,Koki Takahashi,Kotaro Yoshimura,Emiko Aiba-Kojima,Nelson H Tsuno,Keita Inoue,Daisuke Matsumoto,Tomokuni Shigeura,Takahiro Sato,Hirotaka Suga,Harunosuke Kato,Takashi Nagase,Koichi Gonda,Isao Koshima,Koki Takahashi,Kotaro Yoshimura,

Wound fluids, human serum from platelet-poor and platelet-rich plasma (SPPP and SPRP), contain various soluble factors involved in cell growth and proliferation. Levels of cytokines, chemokines, and matrix metalloproteinases (MMPs) in drainage fluids (DFs) harvested from subcutaneous wounds, punctured fluids (PF) from seroma, and SPPP were measured. SPPP and SPRP from four healthy volunteers were also subjected to the analysis. Biochemical profiles of DF reflected the sequential stages of wound healing. Early-phase DF contained high concentrations of basic fibroblast growth factor and platelet-derived growth factor and EGF. The levels of keratinocyte growth factor, interleukin-6, and MMP-8 in DF peaked on days 2-3, while vascular endothelial growth factor, hepatocyte growth factor, interleukin-8, and MMP-1 increased over time during days 0-6. Punctured fluids contained high levels of TGF-beta1, keratinocyte growth factor, vascular endothelial growth factor, hepatocyte growth factor, and MMP-1. Experiments using human adipose-derived stem cells and dermal fibroblasts cultured in media containing various concentrations of DF and fetal bovine serum suggested that for some cell types, DF-contained growth factors that are not obtained from SPRP could be used to supplement or substitute for serum in culture media. SPRP and DF are economical ready-made mixtures of serum and autologous soluble factors, and may be differentially useful for regenerative therapies.

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Publishing Authors By Initials

E Aiba-Kojima,NH Tsuno,K Inoue,D Matsumoto,T Shigeura,T Sato,H Suga,H Kato,T Nagase,K Gonda,I Koshima,K Takahashi,K Yoshimura,E Aiba-Kojima,NH Tsuno,K Inoue,D Matsumoto,T Shigeura,T Sato,H Suga,H Kato,T Nagase,K Gonda,I Koshima,K Takahashi,K Yoshimura,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE: 2007 Jul-Aug

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Wound repair and regeneration : official publicati

VOLUME: 15

Page Numbers: 511-20

Journal Abbreviation:

ISSN: 1067-1927

DAY: 25

MONTH: 07

YEAR: 2007

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9310939

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture.

AFFILIATION: Department of Plastic Surgery, University of Tokyo Hospital, Tokyo, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Wound Repair Regen

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture Related Publications

• A nested case-control study of stomach cancer and serum insulin-like growth factor (IGF)-1, IGF-2 and IGF-binding protein (IGFBP)-3.
• A prospective study of green tea consumption and oral cancer incidence in Japan.
• Beneficial effects of suplatast tosilate (IPD-1151T) in a rat cystitis model induced by intravesical hydrochloric acid.
• Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture.
• Circadian clock genes and photoperiodism: Comprehensive analysis of clock gene expression in the mediobasal hypothalamus, the suprachiasmatic nucleus, and the pineal gland of Japanese Quail under various light schedules.
• Effects of IP-751, ajulemic acid, on bladder overactivity induced by bladder irritation in rats.
• Effects of anaesthesia on the nitrergic pathway during the micturition reflex in rats.
• Follow-up results of 71 patients undergoing metallic stent placement for the treatment of a malignant obstruction of the superior vena cava.
• Functional analysis of active urethral closure mechanisms under sneeze induced stress condition in a rat model of birth trauma.
• Further evidence that major outer membrane proteins homologous to OmpA in Porphyromonas gingivalis stabilize bacterial cells.
• Herpes simplex virus vector-mediated delivery of glial cell line-derived neurotrophic factor rescues erectile dysfunction following cavernous nerve injury.
• Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation.
• Ifi202, an IFN-inducible candidate gene for lupus susceptibility in NZB/W F1 mice, is a positive regulator for NF-kappaB activation in dendritic cells.
• Inhibitory roles of peripheral nitrergic mechanisms in capsaicin-induced detrusor overactivity in the rat.
• Interobserver variation in the histopathological assessment of nonalcoholic steatohepatitis.
• Isoform-specific enhancement of adenylyl cyclase activity by n-alkanols.
• Local effects of antimuscarinics.
• Lower urinary tract symptoms (LUTS) and bladder afferent activity.
• Mortality attributable to cigarette smoking in a cohort study in Japan.
• Neurophysiology of stress urinary incontinence.
• Novel mutations in the GRK1 gene in Japanese patients With Oguchi disease.
• Odor-intensity Coding in the Anterior Piriform Cortex.
• Polypoidal choroidal vasculopathy examined with en face optical coherence tomography.
• Reduction of aluminum toxicity by 2-isopropylmalic acid in the budding yeast Saccharomyces cerevisiae.
• Retinal pigment epithelial tear in polypoidal choroidal vasculopathy.
• Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein.
• Role of noradrenergic pathways in sneeze-induced urethral continence reflex in rats.
• The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder.
• The effect of dendritic cells on the retinal cell transplantation.
• Torquetenovirus infection among northeastern Thai blood donors.