Characterization of Vitamin D insensitive prostate cancer cells.

Characterization of Vitamin D insensitive prostate cancer cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of Vitamin D insensitive prostate cancer cells. Abstract Text:

Adebusola A Alagbala,Michael T Moser,Candace S Johnson,Donald L Trump,Barbara A Foster,

The antitumor effects of 1,25-dihydroxyvitamin D(3) (calcitriol) are being exploited for prevention and treatment of prostate cancer (CaP). These studies examined the antiproliferative effects of calcitriol in primary cell cultures derived from transgenic adenocarcinoma of mouse prostate (TRAMP) mice chronically treated with calcitriol (20 microg/kg) or vehicle 3x/week from 4 weeks-of-age until palpable tumors developed. This is a report on the response of two representative control (Vitamin D naïve, naïve) and calcitriol-treated (Vitamin D insensitive, VDI) cells to calcitriol. VDI cells were less sensitive to calcitriol based on less cell growth inhibition and less inhibition of DNA synthesis as measured by MTT and BrdU incorporation assays. Similarly, VDI cells were less sensitive to growth inhibition by the vitamin analog, 19-nor-1alpha,25-dihydroxyvitamin D(2) (paricalcitol). There was no change in apoptosis following treatment of naïve and VDI cells with calcitriol. Vitamin D receptor (VDR) expression was up-regulated by calcitriol in both naïve and VDI cells. In addition, calcitriol induced the Vitamin D metabolizing enzyme, 24-hydroxylase (cyp24) mRNA and enzyme activity similarly in naïve and VDI cells as measured by RT-PCR and HPLC, respectively. In summary, VDI cells are less responsive to the antiproliferative effects of calcitriol. Understanding Vitamin D insensitivity will further clinical development of Vitamin D compounds for prevention and treatment of CaP.

Characterization of Vitamin D insensitive prostate cancer cells. Publishing Authors By Initials

AA Alagbala,MT Moser,CS Johnson,DL Trump,BA Foster,

For similar polycyclic compounds: steroids: secosteroids: vitamin d research abstracts see: polycyclic compounds: steroids: secosteroids: vitamin d research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of Vitamin D insensitive prostate cancer cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of steroid biochemistry and molecular

VOLUME: 103

Page Numbers: 712-6

Journal Abbreviation: J. Steroid Biochem. Mol. Biol.

ISSN: 0960-0760

DAY: 5

MONTH: 02

YEAR: 2007

Characterization of Vitamin D insensitive prostate cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9015483

Characterization of Vitamin D insensitive prostate cancer cells. Keywords Mesh Terms:

KEYWORDS: Vitamin D

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Characterization of Vitamin D insensitive prostate cancer cells. Information

Substance Name: vitamin D 24-hydroxylase

Registry Number: EC 1.14.-

Grant and Affiliation Information for Characterization of Vitamin D insensitive prostate cancer cells.

AFFILIATION: Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA.

Country: England

AGENCY: United States NCI

GRANT: R01 CA095367-04

ACRONYM: CA

MEDLINETA: J Steroid Biochem Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of Vitamin D insensitive prostate cancer cells Related Publications

• 'Kangaroo gait' in ewes.
• A comprehensive mathematical model of microscopic dose deposition in photodynamic therapy.
• A technique for long-term collection of uterine contents from mares.
• Analyzing the use of race and ethnicity in biomedical research from a local community perspective.
• Biological mechanisms of action of novel C-10 non-acetal trioxane dimers in prostate cancer cell lines.
• Characterization of Vitamin D insensitive prostate cancer cells.
• Clearcut: a fast implementation of relaxed neighbor joining.
• Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization.
• Cooperation and conflict in microbial biofilms.
• Diel timing and frequency of sugar feeding in the mosquito Anopheles gambiae, depending on sex, gonotrophic state and resource availability.
• Effect of an energy reduced high protein red meat diet on weight loss and metabolic parameters in obese women.
• Environmental enrichment decreases the afterhyperpolarization in senescent rats.
• Glucocorticoids, the etiology of obesity and the metabolic syndrome.
• How effective are meal replacements for treating obesity?
• Index-of-refraction-dependent subcellular light scattering observed with organelle-specific dyes.
• Inhibition of arginase I activity by RNA interference attenuates IL-13-induced airways hyperresponsiveness.
• Linear systems analysis of the ciliary steering behavior associated with negative-phototaxis in Chlamydomonas reinhardtii.
• Long-term constant light induces constitutive elevated expression of mPER2 protein in the murine SCN: a molecular basis for Aschoff's rule?
• Long-term exposure of female sheep to physiologic concentrations of estradiol: effects on the onset and maintenance of reproductive function, pregnancy, and social development in female offspring.
• Mammalian photoentrainment: results, methods, and approaches.
• Mass spectrometer characterization of halogen gases in air at atmospheric pressure.
• Multifunctional regulators of cell growth are differentially expressed in anergic murine B cells.
• Mutant p53 in MDA-MB-231 breast cancer cells is stabilized by elevated phospholipase D activity and contributes to survival signals generated by phospholipase D.
• Oppositional defiant disorder toward adults and oppositional defiant disorder toward peers: initial evidence for two separate constructs.
• Photodynamic therapy mediates the oxygen-independent activation of hypoxia-inducible factor 1alpha.
• Regulation of mTOR by phosphatidic acid?
• Solitary myofibroma of the sclera.
• Staphylococcus aureus protein A binding to von Willebrand factor A1 domain is mediated by conserved IgG binding regions.
• T cells and B cells in lupus nephritis.
• Uterine defense mechanisms in the mare: Serum opsonins affecting phagocytosis of Streptococcus zooepidemicus by equine neutrophils.