Characterization of two DNA polymerases from cauliflower inflorescence.

Characterization of two DNA polymerases from cauliflower inflorescence. Research Abstract Details 

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Characterization of two DNA polymerases from cauliflower inflorescence. Abstract Text:

H Fukasawa,M Yamaguchi,M Y Chou,H Matsumoto,A Matsukage,

Two distinct DNA polymerases, A and B, isolated from a rapidly growing apical tissue of cauliflower (Brassica oleracea var. botrytis) inflorescence have been characterized, and compared with DNA polymerases, alpha and beta, from mouse myeloma. Polymerase-A bears a strong resemblance to polymerase-alpha from mammalian cells in all properties examined. The character of polymerase-B is also quite similar to polymerase-beta of mammalian cells in chromatographic elution properties, template-primer utilization, sensitivity to inhibitors, response to KCl or KPi concentration, and other requirements for maximal activity, although it has a higher molecular weight (approx. 78,000) even in the presence of 0.25 M KCl that polymerase-beta (mol. wt. less than or equal to 50,000) of mammalian cells. This type of DNA polymerase has not been reported to exist in the plant system.

Characterization of two DNA polymerases from cauliflower inflorescence. Publishing Authors By Initials

H Fukasawa,M Yamaguchi,MY Chou,H Matsumoto,A Matsukage,

For similar genetic structures: templates, genetic research abstracts see: genetic structures: templates, genetic research

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Characterization of two DNA polymerases from cauliflower inflorescence. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 1167-75

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1980

Characterization of two DNA polymerases from cauliflower inflorescence. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Characterization of two DNA polymerases from cauliflower inflorescence. Keywords Mesh Terms:

KEYWORDS: Templates, Genetic

MESH TERMS: enzymology

Chemical & Substance for Abstract: Characterization of two DNA polymerases from cauliflower inflorescence. Information

Substance Name: DNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.7

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Country: JAPAN

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MEDLINETA: J Biochem

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