Characterization of the OxyR regulon of Neisseria gonorrhoeae.

Characterization of the OxyR regulon of Neisseria gonorrhoeae. Research Abstract Details 

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Characterization of the OxyR regulon of Neisseria gonorrhoeae. Abstract Text:

Kate L Seib,Hsing-Ju Wu,Yogitha N Srikhanta,Jennifer L Edwards,Megan L Falsetta,Amanda J Hamilton,Tina L Maguire,Sean M Grimmond,Michael A Apicella,Alastair G McEwan,Michael P Jennings,

OxyR regulates the expression of the majority of H(2)O(2) responses in Gram-negative organisms. In a previous study we reported the OxyR-dependent derepression of catalase expression in the human pathogen Neisseria gonorrhoeae. In the present study we used microarray expression profiling of N. gonorrhoeae wild-type strain 1291 and an oxyR mutant strain to define the OxyR regulon. In addition to katA (encoding catalase), only one other locus displayed a greater than two-fold difference in expression in the wild type : oxyR comparison. This locus encodes an operon of two genes, a putative peroxiredoxin/glutaredoxin (Prx) and a putative glutathione oxidoreductase (Gor). Mutant strains were constructed in which each of these genes was inactivated. A previous biochemical study in Neisseria meningitidis had confirmed function of the glutaredoxin/peroxiredoxin. Assay of the wild-type 1291 cell free extract confirmed Gor activity, which was lost in the gor mutant strain. Phenotypic analysis of the prx mutant strain in H(2)O(2) killing assays revealed increased resistance, presumably due to upregulation of alternative defence mechanisms. The oxyR, prx and gor mutant strains were deficient in biofilm formation, and the oxyR and prx strains had decreased survival in cervical epithelial cells, indicating a key role for the OxyR regulon in these processes.

Characterization of the OxyR regulon of Neisseria gonorrhoeae. Publishing Authors By Initials

KL Seib,HJ Wu,YN Srikhanta,JL Edwards,ML Falsetta,AJ Hamilton,TL Maguire,SM Grimmond,MA Apicella,AG McEwan,MP Jennings,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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MEDLINE DATE:

Characterization of the OxyR regulon of Neisseria gonorrhoeae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular microbiology

VOLUME: 63

Page Numbers: 54-68

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 27

MONTH: 11

YEAR: 2006

Characterization of the OxyR regulon of Neisseria gonorrhoeae. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Characterization of the OxyR regulon of Neisseria gonorrhoeae. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: physiology

Chemical & Substance for Abstract: Characterization of the OxyR regulon of Neisseria gonorrhoeae. Information

Substance Name: oxyR protein, E coli

Registry Number: 0

Grant and Affiliation Information for Characterization of the OxyR regulon of Neisseria gonorrhoeae.

AFFILIATION: School of Molecular and Microbial Sciences and Centre for Metals in Biology, The University of Queensland, Brisbane 4072, Qld, Australia.

Country: England

AGENCY: United States NIAID

GRANT: AI38399

ACRONYM: AI

MEDLINETA: Mol Microbiol

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ACCESSION NUMBER: AE004969

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