Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection.

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Abstract Text:

Jarlath E Nally,Julian P Whitelegge,Sara Bassilian,David R Blanco,Michael A Lovett,

Pathogenic Leptospira species adapt to a wide range of environmental conditions during disease transmission and infection. While the proteome of in vitro cultivated Leptospira has been characterized in several studies to date, relatively little is known of the proteome as expressed by Leptospira during disease processes. Isolates of Leptospira obtained from patients suffering the severe pulmonary form of leptospirosis cause acute lethal infection in guinea pigs and chronic asymptomatic infection in rats. Recent studies have demonstrated that protein and lipopolysaccharide constituents of Leptospira recovered from acutely infected guinea pig tissue differ from that of Leptospira in chronically infected rat tissue and in vitro cultivated Leptospira (J. E. Nally, E. Chow, M. C. Fishbein, D. R. Blanco, and M. A. Lovett, Infect. Immun. 73:3251-3260, 2005). In the current study, the proteome of Leptospira expressed during disease processes was characterized relative to that of in vitro cultivated Leptospira (IVCL) after enrichment for hydrophobic membrane proteins with Triton X-114. Protein samples were separated by two-dimensional gel electrophoresis, and antigens expressed during infection were identified by immunoblotting with monospecific antiserum and convalescent rat serum in addition to mass spectrometry. Results suggest a significant increase in the expression of the outer membrane protein Loa22 during acute infection of guinea pigs relative to other outer membrane proteins, whose expression is generally diminished relative to expression in IVCL. Significant amounts of LipL32 are also expressed by Leptospira during acute infection of guinea pigs.

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Publishing Authors By Initials

JE Nally,JP Whitelegge,S Bassilian,DR Blanco,MA Lovett,

For similar proteins: proteome research abstracts see: proteins: proteome research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 766-73

Journal Abbreviation: Infect. Immun.

ISSN: 0019-9567

DAY: 13

MONTH: 11

YEAR: 2006

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Keywords Mesh Terms:

KEYWORDS: Proteome

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection.

AFFILIATION: Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA. Jarlath.Nally@ucd.ie

Country: United States

AGENCY: United States NIAID

GRANT: AI056258

ACRONYM: AI

MEDLINETA: Infect Immun

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection Related Publications

• An angiotensin converting enzyme haplotype predicts survival in patients with end stage renal disease.
• Anthrax of the eye.
• Cardiac matrix metalloproteinase-2 expression independently induces marked ventricular remodeling and systolic dysfunction.
• Celiac disease.
• Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection.
• Chromosome segregation control by Escherichia coli ObgE GTPase.
• Inhibition of MMP2/MMP9 after spinal cord trauma reduces apoptosis.
• Nucleic acid synthesis during differentiation of Blastocladiella emersonii. NASA CR-673.
• Nutrient canals; a roentgenographic study.
• QUACKERY, MIRACLE HEALING AND MEDICAL CULTS.
• RecA-independent recombination is efficient but limited by exonucleases.
• Replication arrest-stimulated recombination: Dependence on the RecA paralog, RadA/Sms and translesion polymerase, DinB.
• Roentgenographic technic for anterior interproximal region.
• The varieties of pathways to dysfluent reading: comparing subtypes of children with dyslexia at letter, word, and connected text levels of reading.
• The venous manifestations of pulse wave encephalopathy: windkessel dysfunction in normal aging and senile dementia.
• Thomas Bakewell (1761-1835): madhouse keeper and moral therapist.
• Toward a systems biology of mouse inner ear organogenesis: gene expression pathways, patterns and network analysis.
• Variations in hospital administrative costs.