Characterization of the human CD27 ligand, a novel member of the TNF gene family.

Characterization of the human CD27 ligand, a novel member of the TNF gene family. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of the human CD27 ligand, a novel member of the TNF gene family. Abstract Text:

CD27 is a lymphocyte-specific member of the TNF/NGF-R family that is highly induced on T cells after TCR stimulation, primarily on lymphocytes belonging to the unprimed CD45RA+ subset. However, after prolonged activation both in vivo and in vitro, CD27 expression is gradually switched off. In search of physiologic signals that influence CD27 expression, we found that B cell lines can mediate down-regulation of CD27 surface expression on activated T cells via direct cell-to-cell contact and that preincubation of activated T cells with CD27 mAbs prevented this modulation. Based on these observations, we hypothesized that a ligand for CD27L is expressed on B cell lines and that ligand interaction would result in down-modulation of the molecule from the T cell surface. To identify this putative CD27L, mAbs were raised against the Burkitt cell line Ramos and screened for their ability to block down-modulation of CD27 on activated T cells. Using this approach, we isolated a mAb, designated 2F2, that inhibits CD27 down-regulation in a dose-dependent manner. We demonstrate that this mAb recognizes CD27L as it 1) reacted to mouse fibroblasts expressing the recently cloned CD27L, a novel surface-expressed member of the TNF gene family, and 2) prevented binding of a soluble CD27-Fc construct to a CD27L expressing cell line. Down-regulation of CD27 from the T cell surface by recombinant CD27L was shown to be at least partially caused by release of soluble CD27. The 2F2 mAb enabled us to begin to analyze the biochemical properties, tissue distribution, and function of the CD27L on human cells. From cell lysates of 125I surface-labeled Burkitt cells a complex immunoprecipitation pattern with dominant bands of 29, 55, and 122 to 127 kDa was obtained using the 2F2 mAb. Phenotypical analyses showed that freshly isolated lymphocytes lacked CD27L expression, but that expression of the molecule could be induced after in vitro stimulation of T and B cells. No expression was found on cells of the myeloid lineage or on endothelial cells. Finally, blocking of naturally expressed CD27L by the 2F2 mAb exerted a potent inhibitory effect on the proliferation of T cells in response to allogeneic B cells and PHA. The data presented in this paper identify CD27 and its ligand as potentially important structures involved in cellular interactions between T and B lymphocytes.

Characterization of the human CD27 ligand, a novel member of the TNF gene family. Publishing Authors By Initials

For similar peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of the human CD27 ligand, a novel member of the TNF gene family. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 152

Page Numbers: 1762-73

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 15

MONTH: Feb

YEAR: 1994

Characterization of the human CD27 ligand, a novel member of the TNF gene family. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

Characterization of the human CD27 ligand, a novel member of the TNF gene family. Keywords Mesh Terms:

KEYWORDS: Tumor Necrosis Factor-alpha

MESH TERMS: genetics

Chemical & Substance for Abstract: Characterization of the human CD27 ligand, a novel member of the TNF gene family. Information

Substance Name: Tumor Necrosis Factor-alpha

Registry Number: 0

Grant and Affiliation Information for Characterization of the human CD27 ligand, a novel member of the TNF gene family.

AFFILIATION: Department of Clinical (Viro-)Immunology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of the human CD27 ligand, a novel member of the TNF gene family Related Publications

• A fingerprint left by cytomegalovirus infection in the human T cell compartment.
• Adapting UASB technology for sewage treatment in Palestine and Jordan.
• Adequate synapse formation between leukemic B cells and effector T cells following stimulation with artificial TCR ligands.
• Biodistribution and tumor uptake of [67Ga]chlorogallium-tetraoctadecyloxy phthalocyanine and its sulfonation products in tumor bearing C3H mice.
• CD27 Defines Phenotypically and Functionally Different Human NK Cell Subsets.
• CD27: marker and mediator of T-cell activation?
• CD70 represents the human ligand for CD27.
• Characterization of murine CD70, the ligand of the TNF receptor family member CD27.
• Characterization of the human CD27 ligand, a novel member of the TNF gene family.
• Cytotoxic human CD4(+) T cells.
• Differential Regulation of Human IL-7 Receptor {alpha} Expression by IL-7 and TCR Signaling.
• Engagement of CD27 with its ligand CD70 provides a second signal for T cell activation.
• Expression of the murine CD27 ligand CD70 in vitro and in vivo.
• Grouping effects in flash-induced perceptual fading.
• Human Cytomegalovirus Infection Induces a Rapid and Sustained Change in the Expression of NK Cell Receptors on CD8+ T Cells.
• Infarcted heart uptake and biodistribution of radiolabelled anti-myosin monoclonal antibody in rat and dog myocardial infarct models.
• Lethal T cell immunodeficiency induced by chronic costimulation via CD27-CD70 interactions.
• Oxidation and reactivity of nitrogen- and phosphorus-doped heterofullerenes.
• Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine.
• Sterol metabolism. IV. Microbial disposition of 5beta-cholestan-3beta-ol.
• Synthesis, receptor binding and target-tissue uptake of carbon-11-labeled carbamate derivatives of estradiol and hexestrol.
• Targeting gastrin-releasing peptide receptors of prostate cancer cells for photodynamic therapy with a phthalocyanine-bombesin conjugate.
• The size and phenotype of virus-specific T cell populations is determined by repetitive antigenic stimulation and environmental cytokines.
• Thermophilic treatment of acidified and partially acidified wastewater using an anaerobic submerged MBR: Factors affecting long-term operational flux.
• [(18)F]Fluorinated estradiol derivatives for oestrogen receptor imaging: impact of substituents, formulation and specific activity on the biodistribution in breast tumour-bearing mice.